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Gene info
SESTRIN2, also known as Hi95, is a member of the sestrin family of PA26-related proteins and is induced following DNA damage or oxidative stress (1). SESTRIN2 and the related protein SESTRIN1 interact with the NRF2 suppressor KEAP1, the autophagy substrate p62 and the ubiquitin ligase RBX1 to protect cells against oxidative stress by activating NRF2 and promoting the p62-dependent autophagic degradation of KEAP1 (2). SESTRIN2 also negatively regulates TOR signaling by activating AMP-activated protein kinase (AMPK) and TSC2 phosphorylation (3), thereby protecting cells against energetic stress-induced death (4).
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Gene names
SESTRIN-2, SES2, SEST2, SESN2, HI95
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Antibodie s label
unlabelled serum
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Purity
SESTRIN2 antibody is affinity chromatography purified via peptide column.
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Ab raised in
Rabbit
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Type
Polyclonal
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Immunoglobulin
IgG
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Spicific to
SESTRIN2 antibody is human, mouse and rat reactive. At least two isoforms of SESTRIN2 are known to exist; this antibody will detect both isoforms. SESTRIN2 antibody is predicted to not cross-react with SESTRIN1.
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Antigen
SESTRIN2 antibody was made against a 19 amino acid peptide near the center of human SESTRIN2.
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Immuno application
ELISA, Western Blot, immuno-histo-chemistry, immuno fluorescence
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Antibody advice
SESTRIN2 antibody can be used for detection of SESTRIN2 by Western blot at 1 - 2 ug/ml. Antibody can also be used for immunohistochemistry starting at 5 ug/mL. For immunofluorescence start at 20 ug/mL.
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Suspention pH azide
SESTRIN2 antibody is supplied in PBS containing 0.02% sodium azide.
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Keep at
SESTRIN2 antibody can be stored at 4 grades C for three months and -20 grades C, stable for up to one year.
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Works with
Human, Mouse, Rat
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Omim nr
13899299
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Ncbi nr
NP_113647
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KDa
Predicted: 54 kDa Observed: 53 kDa
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Vial with antibody
in solution
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Quantity per ml
1 mg/mL
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NCBI GENE nr
83667
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GENE symbol
SESN2
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Antigene name
sestrin 2
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Source
homo sapiens
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Protein nr
P58004
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
