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Gene info
N-Oct-3 (POU3F2) is a protein belonging to a large family of transcription factors that bind to the octameric DNA sequence ATGCAAAT. Most of these proteins share a highly homologous region, referred to as the POU domain, which occurs in several mammalian transcription factors, including the octamer-binding proteins Oct1 (POU2F1) and Oct2 (POU2F2), and the pituitary protein Pit1 (PIT1). Class III POU genes are expressed predominantly in the CNS. It is likely that CNS-specific transcription factors such as these play an important role in mammalian neurogenesis by regulating their diverse patterns of gene expression. N-Oct-3 is a protein belonging to a large family of transcription factors that bind to the octameric DNA sequence ATGCAAAT. Most of these proteins share a highly homologous region, referred to as the POU domain, which occurs in several mammalian transcription factors, including the octamer-binding proteins Oct1 (POU2F1; MIM 164175) and Oct2 (POU2F2; MIM 164176), and the pituitary protein Pit1 (PIT1; MIM 173110). Class III POU genes are expressed predominantly in the CNS. It is likely that CNS-specific transcription factors such as these play an important role in mammalian neurogenesis by regulating their diverse patterns of gene expression.[supplied by OMIM].
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Gene names
POU3F2, BRN2, OCT7, OTF7, OTF-7, POUF3, brn-2, oct-7, N-Oct3
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Antibodie s label
unlabelled serum
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Purity
Antibody is purified by peptide affinity chromatography method.
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Ab raised in
Rabbit
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Type
Polyclonal
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Antigen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human POU3F2.
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Immuno application
ELISA, Western Blot
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Antibody advice
POU3F2 antibody can be used for detection of POU3F2 by ELISA at 1:62500. POU3F2 antibody can be used for detection of POU3F2 by western blot at 0.25 ug/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
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Suspention pH azide
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.
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Keep at
For short periods of storage (days) store at 4 grades C. For longer periods of storage, store POU3F2 antibody at -20 grades C. As with any antibody avoid repeat freeze-thaw cycles.
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Works with
Human, Mouse, Rat, Dog
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Omim nr
51702521
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Ncbi nr
NP_005595
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KDa
47 kDa
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Vial with antibody
Lyophilized
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Quantity per ml
1 mg/ml
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NCBI GENE nr
5454
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GENE symbol
POU3F2
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Antigene name
POU class 3 homeobox 2
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Source
Homo sapiens
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Protein nr
P20265
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
