anti-POU1F1 Antibody
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Catalog number25-055
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PricePlease ask
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Size50 ug
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Gene infoPIT1 is a pituitary-specific transcription factor responsible for pituitary development and hormone expression in mammals and is a member of the POU family of transcription factors that regulate mammalian development. The POU family is so named because the first 3 members identified were PIT1 and OCT1 of mammals, and Unc-86 of C. elegans. PIT1 contains 2 protein domains, termed POU-specific and POU-homeo, which are both necessary for high affinity DNA binding on genes encoding growth hormone and prolactin. PIT1 is also important for regulation of the genes encoding prolactin and thyroid-stimulating hormone beta subunit by thyrotropin-releasing hormone and cyclic AMP. This gene encodes a member of the POU family of transcription factors that regulate mammalian development. The protein regulates expression of several genes involved in pituitary development and hormone expression. Mutations in this genes result in combined pituitary hormone deficiency. Multiple transcript variants encoding different isoforms have been found for this gene.
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Gene namesPOU1F1, GHF-1, PIT1, Pit-1, Pit-1 beta, CPHD1, POU1F1a
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Antibodie s labelunlabelled serum
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PurityAntibody is purified by peptide affinity chromatography method.
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Ab raised inRabbit
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TypePolyclonal
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AntigenAntibody produced in rabbits immunized with a synthetic peptide corresponding a region of human POU1F1.
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Immuno applicationELISA, Western Blot
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Antibody advicePOU1F1 antibody can be used for detection of POU1F1 by ELISA at 1:312500. POU1F1 antibody can be used for detection of POU1F1 by western blot at 1 ug/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
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Suspention pH azideAntibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.
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Keep atFor short periods of storage (days) store at 4 grades C. For longer periods of storage, store POU1F1 antibody at -20 grades C. As with any antibody avoid repeat freeze-thaw cycles.
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Works withHuman, Dog
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Omim nr4505955
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Ncbi nrNP_000297
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KDa33 kDa
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Vial with antibodyLyophilized
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Quantity per ml1 mg/ml
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NCBI GENE nr5449
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GENE symbolPOU1F1
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Antigene namePOU class 1 homeobox 1
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SourceHomo sapiens
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Protein nrP28069
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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PropertiesIf you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolPOU1F1
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Short nameanti-POU1F1 Antibody
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TechniqueAntibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for
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Alternative nameAntibody toPOU1F1 (antibody to-)
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Alternative techniqueantibodies
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Gene info
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Identity
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Gene
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Long gene namePOU class 1 homeobox 1
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Synonyms gene
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Synonyms gene name
- POU domain class 1, transcription factor 1
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1993-01-12
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- POU class homeoboxes and pseudogenes
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
