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Gene info
POLR2I is a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with two other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. POLR2I has two zinc finger motifs with conserved cysteines and the subunit does possess zinc binding activity. Western blots using two different antibodies against two unique regions of this protein target confirm the same apparent molecular weight in our tests.This gene encodes a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with two other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. The product of this gene has two zinc finger motifs with conserved cysteines and the subunit does possess zinc binding activity. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
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Gene names
POLR2I, RPB9, hRPB14.5
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Antibodie s label
unlabelled serum
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Purity
Antibody is purified by peptide affinity chromatography method.
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Ab raised in
Rabbit
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Type
Polyclonal
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Antigen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human POLR2I.
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Immuno application
ELISA, Western Blot
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Antibody advice
POLR2I antibody can be used for detection of POLR2I by ELISA at 1:312500. POLR2I antibody can be used for detection of POLR2I by western blot at 1 ug/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
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Suspention pH azide
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.
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Keep at
For short periods of storage (days) store at 4 grades C. For longer periods of storage, store POLR2I antibody at -20 grades C. As with any antibody avoid repeat freeze-thaw cycles.
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Works with
Human, Mouse, Rat
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Omim nr
5453930
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Ncbi nr
NP_006224
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KDa
14 kDa
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Vial with antibody
Lyophilized
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Quantity per ml
1 mg/ml
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NCBI GENE nr
5438
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GENE symbol
POLR2I
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Antigene name
polymerase (RNA) II (DNA directed) polypeptide I, 14.5kDa
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Source
Homo sapiens
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Protein nr
P36954
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
