-
Gene info
PAK4 Antibody: The p21-activated kinases (PAKs) are serine-threonine kinases that bind to the active forms of Cdc42 and Rac. They are divided into two groups, the first of which include PAK1, 2 and 3, and can be activated by Cdc42/Rac binding. Group 1 PAKs contain an autoinhibitory domain whose activity is regulated by Cdc42/Rac binding. The group 1 PAKs are known to be involved in cellular processes such as gene transcription, apoptosis, and cell morphology and motility. Much less is known about the second group, which includes PAK4, 5 and 6. These proteins are not activated by Cdc42/Rac binding. PAK4 was initially identified as a novel effector of Cdc42Hs. Co-expression of PAK4 and Cdc42Hs resulted in induction of filopodia and actin polymerization, showing that it is involved in cytoskeletal reorganization. Other experiments have shown PAK4 to be essential for embryonic viability and proper neuronal development. PAK4 has also been implicated in anchorage-independent growth of tumor cells and is required for activation of several cancer prosurvival pathways.
-
Gene names
PAK4 Antibody: KIAA1142, p21-activated kinase 4, PAK-4
-
Antibodie s label
unlabelled serum
-
Purity
PAK4 Antibody is affinity chromatography purified via peptide column.
-
Ab raised in
Rabbit
-
Type
Polyclonal
-
Immunoglobulin
IgG
-
Antigen
PAK4 antibody was made against a 13 amino acid synthetic peptide from near the center of human PAK4.
-
Immuno application
ELISA, Western Blot, immuno-histo-chemistry, immuno fluorescence
-
Antibody advice
PAK4 antibody can be used for the detection of PAK4 by Western blot at 1 - 2 ug/mL. Antibody can also be used for immunohistochemistry starting at 10 ug/mL. For immunofluorescence start at 20 ug/mL.
-
Suspention pH azide
PAK4 Antibody is supplied in PBS containing 0.02% sodium azide.
-
Keep at
PAK4 antibody can be stored at 4 grades C for three months and -20 grades C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
-
Works with
Human
-
Omim nr
5031975
-
Ncbi nr
NP_005875
-
Vial with antibody
in solution
-
Quantity per ml
1 mg/mL
-
NCBI GENE nr
10298
-
GENE symbol
PAK4
-
Antigene name
p21 protein (Cdc42/Rac)-activated kinase 4
-
Source
Homo sapiens
-
Protein nr
O96013
-
-
-
-
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
-
Properties
If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
-
French translation
anticorps
