anti-Osteoprotegerin Antibody
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Catalog number
XP-5253
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Price
Please ask
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Size
0.1 mg
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Gene info
Osteoprotegerin (OPG) is a member of the TNF receptor superfamily. This protein is an osteoblast-secreted decoy receptor that functions as a negative regulator of bone resorption. This protein specifically binds to its ligand, osteoprotegerin ligand (TNFSF11/OPGL), both of which are key extracellular regulators of osteoclast development. Studies of the mouse counterpart also suggest this protein and its ligand play a role in lymph-node organogenesis and vascular calcification. Alternatively spliced transcript variants of the gene have been reported, but their full length nature has not been determined.
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Gene names
OPG, TR1, OCIF, OPG, Tumor necrosis factor receptor superfamily member 11B, Osteoclastogenesis inhibitory factor,
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Antibodie s label
unlabelled serum
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Purity
Anti-hOPG specific antibody was purified by affinity chromatography employing immobilized hOPG matrix.
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Ab raised in
Rabbit
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Type
Polyclonal
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Antigen
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hOPG (human Osteoprotegrin).
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Immuno application
ELISA, Western Blot
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Antibody advice
ELISA:To detect hOPG by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 ug/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hOPG. Sandwich:To detect hOPG by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 ug/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human OPG (XP-5253Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hOPG.Western Blot:To detect hOPG by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hOPG is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
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Keep at
Osteoprotegerin antibody is stable for at least 2 years from date of receipt at -20 grades C. The reconstituted antibody is stable for at least two weeks at 2-8 grades C. Frozen aliquots are stable for at least 6 months when stored at -20 grades C. Avoid repeated freeze-thaw cycles.
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Works with
Human
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Omim nr
322510101
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Ncbi nr
O00300
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Vial with antibody
Lyophilized
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NCBI GENE nr
4982
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GENE symbol
TNFRSF11B
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Antigene name
tumor necrosis factor receptor superfamily, member 11b
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Source
Homo sapiens
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Protein nr
O00300
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
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Gene target
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Gene symbol
TNFRSF11B
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Short name
anti-Osteoprotegerin Antibody
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Technique
Antibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for
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Alternative name
Antibody toOsteoprotegerin (antibody to-)
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Alternative technique
antibodies
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Gene info
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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