anti-NSUN2 Antibody
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Catalog number26-350
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PricePlease ask
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Size50 ug
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Gene infoMaturation of cytoplasmic tRNAs includes splicing of introns, which are located 1 nucleotide 3-prime from the anticodon in all intron-containing tRNA genes. In tRNA-leu (CAA), the first position of the anticodon, C34, is converted to 5-methylcytosine, a modification necessary to stabilize the anticodon-codon pairing and correctly translate the mRNA. NSUN2 is a methyltransferase that catalyzes the intron-dependent formation of 5-methylcytosine at C34 of tRNA-leu (CAA).Maturation of cytoplasmic tRNAs includes splicing of introns, which are located 1 nucleotide 3-prime from the anticodon in all intron-containing tRNA genes. In tRNA-leu (CAA), the first position of the anticodon, C34, is converted to 5-methylcytosine, a modification necessary to stabilize the anticodon-codon pairing and correctly translate the mRNA. NSUN2 encodes a methyltransferase that catalyzes the intron-dependent formation of 5-methylcytosine at C34 of tRNA-leu (CAA) (Brzezicha et al., 2006 [PubMed 17071714]).
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Gene namesNSUN2, FLJ20303, MISU, SAKI, TRM4, MRT5
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Antibodie s labelunlabelled serum
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PurityAntibody is purified by peptide affinity chromatography method.
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Ab raised inRabbit
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TypePolyclonal
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AntigenAntibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NSUN2.
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Immuno applicationELISA, Western Blot
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Antibody adviceNSUN2 antibody can be used for detection of NSUN2 by ELISA at 1:62500. NSUN2 antibody can be used for detection of NSUN2 by western blot at 1 ug/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
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Suspention pH azideAntibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.
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Keep atFor short periods of storage (days) store at 4 grades C. For longer periods of storage, store NSUN2 antibody at -20 grades C. As with any antibody avoid repeat freeze-thaw cycles.
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Works withHuman, Mouse, Rat
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Omim nr39995082
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Ncbi nrNP_060225
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KDa86 kDa
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Vial with antibodyLyophilized
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Quantity per ml1 mg/ml
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NCBI GENE nr54888
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GENE symbolNSUN2
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Antigene nameNOP2/Sun RNA methyltransferase family, member 2
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SourceHomo sapiens
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Protein nrQ08J23
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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PropertiesIf you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolNSUN2
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Short nameanti-NSUN2 Antibody
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TechniqueAntibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for
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Alternative nameAntibody toNSUN2 (antibody to-)
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Alternative techniqueantibodies
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Gene info
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Identity
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Gene
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Long gene nameNOP2/Sun RNA methyltransferase 2
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Synonyms gene
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Synonyms gene name
- NOL1/NOP2/Sun domain family, member 2
- NOP2/Sun domain family, member 2
- mental retardation, non-syndromic, autosomal recessive, 5
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2004-08-25
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- NOP2/Sun RNA methyltransferase family
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
