anti-NONO Antibody

• Catalog number
  29-404
• Price
  Please ask
• Size
  100 ug

• Gene info
  NONO is DNA- and RNA binding protein, involved in several nuclear processes. It binds the conventional octamer sequence in double stranded DNA. It also binds single-stranded DNA and RNA at a site independent of the duplex site. It is involved in pre-mRNA splicing and interacts with U5 snRNA. The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs, be involved in DNA unwinding by modulating the function of topoisomerase I/TOP1 and be involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V (D)J recombination and may stabilize paired DNA ends. NONO binds to an enhancer element in long terminal repeats of endogenous intracisternal A particles (IAPs) and activates transcription.
• Gene names
  NONO, P54, NMT55, NRB54, P54NRB
• Antibodie s label
  unlabelled serum
• Purity
  Antibody is purified by protein A chromatography method.
• Ab raised in
  Rabbit
• Type
  Polyclonal
• Antigen
  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NONO.
• Immuno application
  ELISA, Western Blot, immuno-histo-chemistry
• Antibody advice
  NONO antibody can be used for detection of NONO by ELISA at 1:62500. NONO antibody can be used for detection of NONO by western blot at 1.25 ug/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
• Suspention pH azide
  Antibody is lyophilized in PBS buffer with 2% sucrose. Add 100 μL of distilled water. Final antibody concentration is 1 mg/mL.
• Keep at
  For short periods of storage (days) store at 4 grades C. For longer periods of storage, store NONO antibody at -20 grades C. As with any antibody avoid repeat freeze-thaw cycles.
• Works with
  Human, Dog
• Omim nr
  34932414
• Ncbi nr
  NP_031389
• KDa
  52 kDa
• Vial with antibody
  Lyophilized
• Quantity per ml
  1 mg/ml
• NCBI GENE nr
  4841
• GENE symbol
  NONO
• Antigene name
  non-POU domain containing, octamer-binding
• Source
  Homo sapiens
• Protein nr
  Q15233
• Picture
• Pdf tech file
• More antibodies
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Properties
  If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  NONO  
• Gene symbol
  NONO
• Short name
  anti-NONO Antibody
• Technique
  Antibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for
• Alternative name
  Antibody toNONO (antibody to-)
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:7871
• Gene
  NONO
• Long gene name
  non-POU domain containing octamer binding
• Synonyms gene name
  • non-POU-domain-containing, octamer-binding
• Synonyms
  • NRB54
  • NMT55
  • P54NRB
  • P54
  • PPP1R114
• Synonyms name
  • Nuclear RNA-binding protein, 54-kD
  • non-Pou domain-containing octamer (ATGCAAAT) binding protein
  • protein phosphatase 1, regulatory subunit 114
• GenBank acession
  • L14599
• Locus
  Xq13.1
• Discovery year
  1992-02-06
• Entrez gene record
  4841
• Pubmed identfication
  • 8371983
  • 9360842
• RefSeq identity
  • NM_007363
• Classification
  • Protein phosphatase 1 regulatory subunits
  • RNA binding motif containing
• VEGA ID
  OTTHUMG00000021798

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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