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Gene info
The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death. Increased membrane surface expression of the NR2A subunit of the receptor has been associated synaptic plasticity.
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Gene names
NR2A, GluN2A, NMDAR2A, N-methyl D-aspartate receptor subtype 2A
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Antibodie s label
unlabelled serum
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Purity
Affinity Purified
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Ab raised in
Rabbit
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Type
Polyclonal
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Antigen
NMDA NR2A polyclonal antibody was made against a C-terminal fusion protein of the NR2A subunit of the NMDAR.
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Immuno application
Western Blot, immuno-histo-chemistry, immuno precipitation
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Antibody advice
NMDA NR2A Subunit antibody is specific for the 180k NR2A subunit in Western blots of most brain regions. Immunolabeling blocked by pre-adsorption of antibody with the C-terminal fusion protein of the NR2A subunit that was used to generate the antibody. No reactivity towards the NR2B subunit of the NMDAR. Applications include Dot Blots (DB), Immunohistochemistry (IHC), Immunoprecipitation (IP) and Western Blots (WB). Human, mouse, and rat have 100% amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for IHC, DB and WB, and 3 ug/200 ug lysate will quantitatively precipitate all NMDAR 2A for IP.
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Keep at
NMDA NR2A Subunit antibody can be stored at -20 grades C. After reconstitution in 50 μl PBS, the antibody should be aliquot and stored at -20 grades C and is stable at -20 grades C for at least 1 year. Ambient shipment is suitable for both international and domestic.
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Works with
Human, Rat
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Omim nr
3915771
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Ncbi nr
Q00959
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KDa
180
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Vial with antibody
Lyophilized
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NCBI GENE nr
24409
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GENE symbol
Grin2a
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Antigene name
glutamate receptor, ionotropic, N-methyl D-aspartate 2A
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Source
Rattus norvegicus
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Protein nr
Q00959
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
