anti-MECP2 Antibody

• Catalog number
  49-305
• Price
  Please ask
• Size
  0.05 mg

• Gene info
  DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD). The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA. MBD2 and MBD4 (also known as MED1) were later found to colocalize with foci of heavily methylated satellite DNA and believed to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. MBD4 have homology to bacterial base excision repair DNA N-glycosylases/lyases. In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract.
• Gene names
  MECP2, AUTSX3, Methyl-CpG-binding protein 2, MRX16, MRX79, MRXSL, RTT, RTS, MeCp-2 protein, RS, MRXS13, PPMX
• Antibodie s label
  unlabelled serum
• Purity
  Protein G Column
• Ab raised in
  Rabbit
• Type
  Polyclonal
• Immunoglobulin
  IgG
• Antigen
  MECP2 antibody was made against amino acids 11 - 25 of MECP2 (Human).
• Immuno application
  Western Blot, immuno-histo-chemistry
• Antibody advice
  MECP2 antibody can be used in ELISA starting at 1:10000, Western Blot starting at 1:500 - 1:2000, immunohistochemistry starting at 1:50, immunofluorescence starting at 1:200 - 1:1000, and flow cytometry starting at 1:200 - 1:400.
• Suspention pH azide
  PBS, 0.2% gelatin, 0.05% sodium azide.
• Keep at
  MECP2 antibody can be stored short term 4 grades C. For long term storage aliquot and store at -20 grades C. As with all antibodies avoid freeze/thaw cycles.
• Works with
  Human
• Omim nr
  1708973
• Ncbi nr
  P51608
• Vial with antibody
  in solution
• NCBI GENE nr
  4204
• GENE symbol
  MECP2
• Antigene name
  methyl CpG binding protein 2 (Rett syndrome)
• Source
  Homo sapiens
• Protein nr
  P51608
• Picture
• Pdf tech file
• More antibodies
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Properties
  If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  MECP2  
• Gene symbol
  MECP2
• Short name
  anti-MECP2 Antibody
• Technique
  Antibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for
• Alternative name
  Antibody toMECP2 (antibody to-)
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:6990
• Gene
  MECP2
• Long gene name
  methyl-CpG binding protein 2
• Synonyms gene
  • RTT
  • MRX16
  • MRX79
• Synonyms gene name
  • mental retardation, X-linked 16
  • mental retardation, X-linked 79
  • Rett syndrome
  • methyl CpG binding protein 2 (Rett syndrome)
  • methyl CpG binding protein 2
• GenBank acession
  • AF158180
• Locus
  Xq28
• Discovery year
  1996-09-03
• Entrez gene record
  4204
• Pubmed identfication
  • 1606614
  • 10508514
• RefSeq identity
  • NM_004992
• Classification
  • Methyl-CpG binding domain containing
• VEGA ID
  OTTHUMG00000024229
• Locus Specific Databases
  • RettBASE
  • Global Variome shared LOVD
  • LRG_764

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Similar products