anti-Leptin Antibody [G1_4B6.3]

• Catalog number
  38-235
• Price
  Please ask
• Size
  0.5 mg

• Gene info
  Leptin plays a critical role in the regulation of body weight by inhibiting food intake and stimulating energy expenditure. Defects in Leptin production cause severe hereditary obesity in rodents and humans. In addition to its effects on body weight, leptin has a variety of other functions, including the regulation of hematopoiesis, angiogenesis, wound healing, and the immune and inflammatory response. The Leptin gene is the human homolog of the gene (ob) mutant in the mouse 'obese' phenotype.Was product number XP-5712-M
• Gene names
  OB, OBS, Leptin, Obese proteinOB, LEPD
• Antibodie s label
  unlabelled serum
• Purity
  This IgG1K antibody was purified from cell culture by Protein A affinity chromatography.
• Ab raised in
  Mouse
• Type
  Monoclonal
• Monoclonal
  G1_4B6.3
• Antigen
  Leptin antibody is produced in BALB/c mice using highly pure (>98%) recombinant human Leptin as the immunizing antigen.
• Immuno application
  ELISA, Western Blot, NELISAut
• Antibody advice
  Neutralization:To yield one-half maximal inhibition [ND50] of the biological activity of mIL-2 (1.0 ng/mL), a concentration of 10.0-14.0 ug/mL of this antibody is required. ELISA:In a sandwich ELISA (assuming 100 μL/well), a concentration of 4.0-8.0 ug/mL of this antibody will detect at least 32 pg/mL of recombinant murine IL-2 when used with our biotinylated antigen affinity purified anti-murine IL-2 (XP-5183Bt) as the detection antibody at a concentration of at least 1.0-2.0 ug/mL.Western Blot:To detect mIL-2 by Western Blot analysis this antibody can be used at a concentration of 2.0-4.0 ug/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-2 is 1.0-2.0 ng/lane, under reducing conditions and 0.2-0.4 ng/lane, under non-reducing conditions.
• Keep at
  Leptin lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20 grades C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4 grades C when reconstituted.
• Works with
  Human
• Omim nr
  730218
• Ncbi nr
  P41159
• Vial with antibody
  Lyophilized
• NCBI GENE nr
  3952
• GENE symbol
  LEP
• Antigene name
  leptin
• Source
  Homo sapiens
• Protein nr
  P41159
• Picture
• Pdf tech file
• More antibodies
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Properties
  If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• Gene
  Human or mouse Leptin (from Greek λεπτός leptos, "thin") the "satiety hormone", is a hormone made by adipose cells that helps to regulate energy balance by inhibiting hunger. Leptin is opposed by the actions of the hormone ghrelin, the "hunger hormone". Both hormones act on receptors in the arcuate nucleus of the hypothalamus to regulate appetite to achieve energy homeostasis. ELISA kits and peptides and antibodies are available.
• French translation
  anticorps

• Gene target
  Leptin   [G1   4B6  
• Short name
  anti-Leptin Antibody [G1_4B6 3]
• Technique
  Antibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for
• Alternative name
  Antibody toLeptin (antibody to-) [G1_4B6.3]
• Alternative technique
  antibodies

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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