anti-GAPDH Antibody [1D4]

• Catalog number
  50-267
• Price
  Please ask
• Size
  0.1 mL

• Gene info
  Biological Significance: Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) is a metabolic enzyme responsible for catalyzing one step in the glycolytic pathway, the reversible oxidative phosphorylation of glyceraldehyde 3-phosphate. Because GAPDH is a protein expressed in large amounts and which is required at all times for important "house keeping" functions, levels of GAPDH mRNA are often measured and used as standards in studies of mRNA expression. Increasingly, scientists are making use of specific antibodies to GAPDH in comparable studies of levels of protein expression. This antibody can be used as a loading control for western blotting experiments, allowing comparison between the level of this protein and others in a cell or tissue. Apart from a role in glycolysis, GAPDH may have other roles such as in the activation of transcription. GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein, mutations in which cause some forms of Alzheimer's disease, and the polyglutamine tracts of Huntingtin, the protein product aberrant forms of which are causative of Huntington's disease. Associations with actin and tubulin have also been reported. The protein may also have a role in the regulation of apoptosis, and interestingly migrates from the cytoplasm into the nucleus when cells become apoptotic.
• Gene names
  GAPD2, GAPDS, HSD-35, GAPDH-2, GAPD2, GAPDH2, GAPDSORF Names:HSD-35, HSD35, Spermatogenic cell-specific glyceraldehyde 3-phosphate dehydrogenase 2
• Antibodie s label
  unlabelled serum
• Purity
  Total IgM fraction--concentrated culture supernatant
• Ab raised in
  Mouse
• Type
  Monoclonal
• Monoclonal
  1D4
• Immunoglobulin
  IgM
• Spicific to
  Specific for the ~38k Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) protein.
• Antigen
  Extensively purified pig GAPDH.
• Immuno application
  Western Blot, immuno-histo-chemistry
• Keep at
  GAPDH antibody can be stored at -20 grades C and is stable at -20 grades C for at least 1 year.
• Works with
  Human, Mouse, Rat, Bovine, Chicken, Porcine
• Omim nr
  6226817
• Ncbi nr
  O14556
• KDa
  38
• Vial with antibody
  in solution
• NCBI GENE nr
  26330
• GENE symbol
  GAPDHS
• Antigene name
  glyceraldehyde-3-phosphate dehydrogenase, spermatogenic
• Source
  Homo sapiens
• Protein nr
  O14556
• Picture
• Pdf tech file
• More antibodies
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Properties
  If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  GAPDH   [1D4]  
• Gene symbol
  GAPDH-DT, GAPDH, GAPDHS, GAPDHP15
• Short name
  anti-GAPDH Antibody [1D4]
• Technique
  Antibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for
• Alternative name
  Antibody toGAPDH (antibody to-) [1D4]
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:55492
• Gene
  GAPDH-DT
• Long gene name
  GAPDH divergent transcript
• Locus
  12p13.31
• Discovery year
  2021-03-12
• Entrez gene record
  112268089
• Classification
  • Divergent transcripts

Gene info

• Identity
  HGNC:4141
• Gene
  GAPDH
• Long gene name
  glyceraldehyde-3-phosphate dehydrogenase
• Synonyms gene
  • GAPD
• GenBank acession
  • AF261085
• Locus
  12p13.31
• Discovery year
  2001-06-22
• Entrez gene record
  2597
• Pubmed identfication
  • 3170585
• RefSeq identity
  • NM_002046
• VEGA ID
  OTTHUMG00000137379

Gene info

• Identity
  HGNC:24864
• Gene
  GAPDHS
• Long gene name
  glyceraldehyde-3-phosphate dehydrogenase, spermatogenic
• Synonyms gene
  • GAPDS
• Synonyms
  • GAPDH-2
  • GAPD2
• GenBank acession
  • AJ005371
• Locus
  19q13.12
• Discovery year
  2004-02-10
• Entrez gene record
  26330
• Pubmed identfication
  • 10714828
• RefSeq identity
  • NM_014364
• VEGA ID
  OTTHUMG00000182298

Gene info

• Identity
  HGNC:13950
• Gene
  GAPDHP15
• Long gene name
  glyceraldehyde-3-phosphate dehydrogenase pseudogene 15
• Synonyms gene
  • GAPDP15
• Synonyms
  • GAPDH-Lp
  • b55C20.4
• Locus
  6p11.2
• Discovery year
  2003-11-26
• Entrez gene record
  642317
• VEGA ID
  OTTHUMG00000014927

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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