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Gene names
Apt1lg1, Cd95l, Fasl, gld, Tnfsf6, Tumor necrosis factor ligand superfamily member 6, CD95 ligand, CD95-L, CD178, CD95L, Fas-L, Faslg, APT1LG1
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Antibodie s label
Biotin
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Purity
FasL (monoclonal H11) antibody was purified to ≥95% in SDS-PAGE.
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Ab raised in
Rat
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Type
Monoclonal
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Monoclonal
H11
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Immunoglobulin
IgG2a
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Antigen
FasL (monoclonal H11) antibody was made against a synthetic peptide corresponding to amino acids 196-220 of mouse FasL.
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Immuno application
Western Blot, immuno-histo-chemistry, FLOW
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Antibody advice
FasL antibody can be used for the detection of mouse FasL by flow cytometry, immunocytochemistry, and western blot. Method: Transfected cells were plated onto polylysine treated glass slides, fixed and permeabilized in methanol at -20oC for 5 min, then in acetone at -20oC for 30 sec. After 3 washes in PBS, 0.1% BSA, were incubated for 1 hr at room temperature with 20 ug/mL of biotinylated H11 antibody in PBS, 0.1% BSA. After rinsing in PBS, FITC-conjugated streptavidin was added for 30 min, slides washed again in PBS and visualized using a fluorescence microscope. Method: Cells extracts from (2 x 106) cells transfected with a FasL-expression plasmid were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and probed with the monoclonal anti-FasL H11 antibody at 2 ug/mL. Proteins were visualized using a peroxidase-conjugated antibody to rat IgG and a chemiluminescence detection system. Arrow indicates FasL. Method: FasL-transfected 293T cells (5 x 105) were incubated on ice for 30 min in 50 μl FACS buffer (PBS, 5% Fetal calf serum, 0.02% azide) containing 1 ug of FITC-labeled H11 antibody. After washing in FACS buffer, cells were analyzed by flow cytometry. Do not exclude pre-apoptotic cells during data acquisition (see T. Renno, M. Hahne, J. Tschopp and H. R. MacDonald, J. Exp. Med., 183, 431 (1996)). Note: Activated primary T cells do not express high levels of surface FasL! This product is for research use only.
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Suspention pH azide
PBS containing 0.02% sodium azide
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Keep at
FasL antibody can be stored at 4 grades C, stable for one year.
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Omim nr
729462
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Ncbi nr
P41047
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Vial with antibody
in solution
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NCBI GENE nr
14103
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GENE symbol
Fasl
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Antigene name
Fas ligand (TNF superfamily, member 6)
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Source
Mus musculus
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Protein nr
P41047
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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Conjugation
Biotinylated
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French translation
anticorps
