anti-FasL Antibody [H11]
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Catalog numberXA-1001F
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PricePlease ask
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Size100 tests
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Gene namesApt1lg1, Cd95l, Fasl, gld, Tnfsf6, Tumor necrosis factor ligand superfamily member 6, CD95 ligand, CD95-L, CD178, CD95L, Fas-L, Faslg, APT1LG1
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Antibodie s labelFITC
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PurityFasL (monoclonal H11) antibody was purified to ≥95% in SDS-PAGE.
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Ab raised inRat
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TypeMonoclonal
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MonoclonalH11
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ImmunoglobulinIgG2a
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AntigenFasL (monoclonal H11) antibody was made against a synthetic peptide corresponding to amino acids 196-220 of mouse FasL.
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Immuno applicationimmuno-histo-chemistry, FLOW
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Antibody adviceFasL antibody can be used for the detection of mouse FasL by flow cytometry and immunocytochemistry. Method: Transfected cells were plated onto polylysine treated glass slides, fixed and permeabilized in methanol at -20oC for 5 min, then in acetone at -20oC for 30 sec. After 3 washes in PBS, 0.1% BSA, were incubated for 1 hr at room temperature with 20 ug/mL of biotinylated H11 antibody in PBS, 0.1% BSA. After rinsing in PBS, FITC-conjugated streptavidin was added for 30 min, slides washed again in PBS and visualized using a fluorescence microscope. Method: Cells extracts from (2 x 106) cells transfected with a FasL-expression plasmid were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and probed with the monoclonal anti-FasL H11 antibody at 2 ug/mL. Proteins were visualized using a peroxidase-conjugated antibody to rat IgG and a chemiluminescence detection system. Arrow indicates FasL. Method: FasL-transfected 293T cells (5 x 105) were incubated on ice for 30 min in 50 μl FACS buffer (PBS, 5% Fetal calf serum, 0.02% azide) containing 1 ug of FITC-labeled H11 antibody. After washing in FACS buffer, cells were analyzed by flow cytometry. Do not exclude pre-apoptotic cells during data acquisition (see T. Renno, M. Hahne, J. Tschopp and H. R. MacDonald, J. Exp. Med., 183, 431 (1996)). Note: Activated primary T cells do not express high levels of surface FasL! This product is for research use only.
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Suspention pH azidePBS containing 0.02% sodium azide
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Keep atFasL antibody can be stored at 4 grades C, stable for one year.
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Omim nr729462
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Ncbi nrP41047
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Vial with antibodyin solution
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NCBI GENE nr14103
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GENE symbolFasl
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Antigene nameFas ligand (TNF superfamily, member 6)
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SourceMus musculus
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Protein nrP41047
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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PropertiesIf you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolHSPB8
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Short nameanti-FasL Antibody [H11]
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TechniqueAntibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for
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Alternative nameAntibody toFasL (antibody to-) [H11]
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Alternative techniqueantibodies
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Gene info
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Identity
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Gene
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Long gene nameheat shock protein family B (small) member 8
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Synonyms gene name
- heat shock 27kDa protein 8
- heat shock 22kDa protein 8
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Synonyms
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GenBank acession
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Locus
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Discovery year2004-01-29
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small heat shock proteins
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data