anti-FasL Antibody [H11]

• Catalog number
  XA-1001
• Price
  Please ask
• Size
  0.1 mg

• Gene names
  Apt1lg1, Cd95l, Fasl, gld, Tnfsf6, Tumor necrosis factor ligand superfamily member 6, CD95 ligand, CD95-L, CD178, CD95L, Fas-L, Faslg, APT1LG1
• Antibodie s label
  unlabelled serum
• Purity
  FasL (monoclonal A11) antibody was purified to ≥95% in SDS-PAGE.
• Ab raised in
  Rat
• Type
  Monoclonal
• Monoclonal
  H11
• Immunoglobulin
  IgG2a
• Antigen
  FasL (monoclonal A11) antibody was made against a synthetic peptide corresponding to amino acids 196-220 of mouse FasL.
• Immuno application
  Western Blot, immuno-histo-chemistry, FLOW
• Antibody advice
  FasL antibody can be used for the detection of mouse FasL by flow cytometry, immunocytochemistry, and western blot. Method: Transfected cells were plated onto polylysine treated glass slides, fixed and permeabilized in methanol at -20oC for 5 min, then in acetone at -20oC for 30 sec. After 3 washes in PBS, 0.1% BSA, were incubated for 1 hr at room temperature with 20 ug/mL of biotinylated H11 antibody in PBS, 0.1% BSA. After rinsing in PBS, FITC-conjugated streptavidin was added for 30 min, slides washed again in PBS and visualized using a fluorescence microscope. Method: Cells extracts from (2 x 106) cells transfected with a FasL-expression plasmid were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and probed with the monoclonal anti-FasL H11 antibody at 2 ug/mL. Proteins were visualized using a peroxidase-conjugated antibody to rat IgG and a chemiluminescence detection system. Arrow indicates FasL. Method: FasL-transfected 293T cells (5 x 105) were incubated on ice for 30 min in 50 μl FACS buffer (PBS, 5% Fetal calf serum, 0.02% azide) containing 1 ug of FITC-labeled H11 antibody. After washing in FACS buffer, cells were analyzed by flow cytometry. Do not exclude pre-apoptotic cells during data acquisition (see T. Renno, M. Hahne, J. Tschopp and H. R. MacDonald, J. Exp. Med., 183, 431 (1996)). Note: Activated primary T cells do not express high levels of surface FasL! This product is for research use only.
• Suspention pH azide
  PBS containing 0.02% sodium azide
• Keep at
  FasL antibody can be stored at 4 grades C, stable for one year.
• Omim nr
  729462
• Ncbi nr
  P41047
• Vial with antibody
  in solution
• NCBI GENE nr
  14103
• GENE symbol
  Fasl
• Antigene name
  Fas ligand (TNF superfamily, member 6)
• Source
  Mus musculus
• Protein nr
  P41047
• Picture
• Pdf tech file
• More antibodies
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Properties
  If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  FasL   [H11]  
• Gene symbol
  HSPB8
• Short name
  anti-FasL Antibody [H11]
• Technique
  Antibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for
• Alternative name
  Antibody toFasL (antibody to-) [H11]
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:30171
• Gene
  HSPB8
• Long gene name
  heat shock protein family B (small) member 8
• Synonyms gene name
  • heat shock 27kDa protein 8
  • heat shock 22kDa protein 8
• Synonyms
  • H11
  • E2IG1
  • HSP22
  • CMT2L
• GenBank acession
  • AF191017
• Locus
  12q24.23
• Discovery year
  2004-01-29
• Entrez gene record
  26353
• Pubmed identfication
  • 10833516
  • 11085516
• RefSeq identity
  • NM_014365
• Classification
  • Small heat shock proteins
• VEGA ID
  OTTHUMG00000168932
• Locus Specific Databases
  • Inherited Peripheral Neuropathies Mutation Database
  • LRG_249

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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