anti-Dynamin (phospho Ser774) Antibody

• Catalog number
  XPS-1006
• Price
  Please ask
• Size
  0.1 mL

• Gene info
  Dynamin is a member of a group of nerve terminal proteins called dephosphins that regulate synaptic vesicle endocytosis. Cyclin dependent protein kinase 5 phosphorylates dynamin at Ser774 and Ser778 that are the phosphorylation sites on dynamin phosphorylated in vivo. Phosphorylation of these sites on dynamin is thought to play a key role in synaptic vesicle trafficking.
• Gene names
  Dnm, Dnm, B-dynamin,
• Antibodie s label
  unlabelled serum
• Purity
  Affinity Purified
• Ab raised in
  Sheep
• Type
  Polyclonal
• Antigen
  Dynamin (Ser774) polyclonal antibody was made against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho-Ser774 of human, mouse and rat dynamin.
• Immuno application
  Western Blot, immuno fluorescence, immuno-histo-chemistry
• Antibody advice
  Dynamin antibody for phospho-Ser774 dynamin labels the purified protein phosphorylated in vitro by Cdk5 but not PKC (Tan, et al. 2003). The labeling of the dynamin band in Western blots of rat brain was blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide. Sheep anti- Dynamin (Ser774) does not cross react with other purified substrates of CDK5 (e.g. amphiphysin and synapsin). Applications include Immunoblotting (IB), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blots (WB). Human, mouse and rat have 100% amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for IB and WB.
• Suspention pH azide
  100 uL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per mL BSA and 50% glycerol.
• Keep at
  For long term storage -80 grades C is recommended, but shorter term storage at -20 grades C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stock solutions are stable for a minimum of 1 year at -20 grades C.
• Works with
  Human, Mouse, Rat
• Omim nr
  190358918
• Ncbi nr
  P21575
• KDa
  95
• Vial with antibody
  in solution
• NCBI GENE nr
  140694
• GENE symbol
  Dnm1
• Antigene name
  dynamin 1
• Source
  Rattus norvegicus
• Protein nr
  P21575
• Picture
• Pdf tech file
• More antibodies
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Properties
  If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  Dynamin   phospho   Ser774  
• Short name
  anti-Dynamin (phospho Ser774) Antibody
• Technique
  Antibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for
• Alternative name
  Antibody toDynamin (phosphorilated Ser774) (antibody to-)
• Alternative technique
  antibodies

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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