anti-DNTT Antibody
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Catalog number30-788
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Price:
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Size50 ug
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Gene infoDNTT is a template-independent DNA polymerase that catalyzes the addition of deoxynucleotides to the 3'-hydroxyl terminus of oligonucleotide primers. In vivo, DNTT is expressed in a restricted population of normal and malignant pre-B and pre-T lymphocytes during early differentiation, where it generates antigen receptor diversity by synthesizing non-germ line elements (N-regions) at the junctions of rearranged Ig heavy chain and T cell receptor gene segments. This gene is a member of the DNA polymerase type-X family and encodes a template-independent DNA polymerase that catalyzes the addition of deoxynucleotides to the 3'-hydroxyl terminus of oligonucleotide primers. In vivo, the encoded protein is expressed in a restricted population of normal and malignant pre-B and pre-T lymphocytes during early differentiation, where it generates antigen receptor diversity by synthesizing non-germ line elements (N-regions) at the junctions of rearranged Ig heavy chain and T cell receptor gene segments. Alternatively spliced transcript variants encoding different isoforms of this gene have been described.
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Gene namesDNTT, TDT
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Antibodie s labelunlabelled serum
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PurityAntibody is purified by peptide affinity chromatography method.
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Ab raised inRabbit
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TypePolyclonal
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AntigenAntibody produced in rabbits immunized with a synthetic peptide corresponding a region of human DNTT.
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Immuno applicationELISA, Western Blot
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Antibody adviceDNTT antibody can be used for detection of DNTT by ELISA at 1:62500. DNTT antibody can be used for detection of DNTT by western blot at 1 ug/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
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Suspention pH azideAntibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.
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Keep atFor short periods of storage (days) store at 4 grades C. For longer periods of storage, store DNTT antibody at -20 grades C. As with any antibody avoid repeat freeze-thaw cycles.
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Works withHuman, Mouse, Rat
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Omim nr63054850
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Ncbi nrNP_004079
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KDa58 kDa
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Vial with antibodyLyophilized
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Quantity per ml1 mg/ml
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NCBI GENE nr1791
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GENE symbolDNTT
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Antigene nameDNA nucleotidylexotransferase
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SourceHomo sapiens
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Protein nrP04053
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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PropertiesIf you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolDNTT
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Short nameanti-DNTT Antibody
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TechniqueAntibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for
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Alternative nameAntibody toDNTT (antibody to-)
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Alternative techniqueantibodies
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Gene info
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Identity
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Gene
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Long gene nameDNA nucleotidylexotransferase
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Synonyms gene name
- deoxynucleotidyltransferase, terminal
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2001-06-22
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Entrez gene record
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RefSeq identity
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Classification
- DNA polymerases
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data