anti-Brg1 Antibody
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Catalog number79-761
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PricePlease ask
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Size0.1 mg
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Gene infoTranscriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. SMARCA4/BAF190A may promote neural stem cell self-renewal/proliferation by enhancing Notch-dependent proliferative signals, while concurrently making the neural stem cell insensitive to SHH-dependent differentiating cues. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. Acts as a corepressor of ZEB1 to regulate E-cadherin transcription and is required for induction of epithelial-mesenchymal transition (EMT) by ZEB1.
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Gene namesBRG1, SNF2, SWI2, MRD16, RTPS2, BAF190, SNF2L4, SNF2LB, hSNF2b, BAF190A, BRG1
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Antibodie s labelunlabelled serum
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PurityAntibodies were purified by affinity-chromatography using epitope-specific peptide.
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Ab raised inRabbit
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TypePolyclonal
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Spicific toThis antibody detects endogenous level of total Brg1 protein.
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AntigenBrg1 antibody was made against a peptide sequence around aa.701~705 (V-D-A-R-H) derived from Human Brg1.
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Immuno applicationWestern Blot
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Antibody adviceWestern Blot: 1:500~1:1000
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Suspention pH azideAntibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Keep atStore antibody at -20 grades C for up to one year.
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Works withHuman, Mouse, Rat
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Omim nr192807312
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Ncbi nrNP_001122316.1
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KDa185, 200 kDa
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Vial with antibodyin solution
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Quantity per ml1 mg/mL
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NCBI GENE nr6597
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GENE symbolSMARCA4
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Antigene nameSWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4
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SourceHomo sapiens
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Protein nrP51532
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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PropertiesIf you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolACTL6A, SMARCD2, SMARCD3
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Short nameanti-Brg1 Antibody
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TechniqueAntibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for
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Alternative nameAntibody toBrg1 (antibody to-)
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Alternative techniqueantibodies
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Gene info
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Identity
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Gene
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Long gene nameactin like 6A
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2004-07-12
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Actin related proteins
- INO80 complex
- PBAF complex
- BAF complex
- GBAF complex
- SRCAP complex
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameSWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 2
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1998-05-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- PBAF complex
- BAF complex
- GBAF complex
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameSWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 3
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1998-05-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- PBAF complex
- BAF complex
- GBAF complex
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data