GoScript Reverse Transcription Mix, Random Primers

• Catalog number
  A2801
• Price
  Please ask
• Size
  100 réaction

• Ambiant Carboglace
  Dry or Gel Ice
• Description
  A primer is a strand of short nucleic acid sequences (generally about 10 base pairs) that serves as a starting point for DNA synthesis. It is required for DNA replication because the enzymes that catalyze this process, DNA polymerases, can only add new nucleotides to an existing strand of DNA. The polymerase starts replication at the 3'-end of the primer, and copies the opposite strand, Reverse transcription primers are used in PCR but in vivo reverse transcription begins when the viral particle that enters the cytoplasm of a target cell with its reverse transcriptase. The viral RNA genome enters the cytoplasm as part of a nucleoprotein complex that has not been well characterized. The process of reverse transcription generates, in the cytoplasm, a linear DNA via an intricate series of steps. This DNA is collinear with its RNA template, but it contains terminal duplications known as the long terminal repeats (LTRs) that are not present in viral RNA . Extant models for reverse transcription propose that two specialized template switches known as strand-transfer reactions or “jumps” are required to generate the LTRs. The activation of transcription factor subunits is the first step of gene expression, in which a particular segment of DNA is copied into RNA (mRNA) by the enzyme RNA polymerases. Transcription factors, unites and elongations can be RNA and DNA nucleic acids, base pairs of nucleotides . Converting from DNA to RNA is made by enzymatic reactions. During transcription, a DNA sequence is read by an RNA polymerase, which produces a complementary, anti-parallel RNA strand called a primary transcript. Transcriptions are key functions in signal transduction pathways. Signaling ligand binding transcription factors play an important role in transduction cascades.
• Goup
  reverse transcription

• Gene target
  GoScript   Transcription   Random  
• Short name
  GoScript Reverse Transcription , Random Primers
• Technique
  primers, reverse, primer
• Alternative name
  GoScript Reverse Transcription Mix, Random Primers
• Alternative technique
  transctription, pcr

MeSH Data

• Name
  Amplified Fragment Length Polymorphism Analysis
• Concept
  Scope note: The detection of RESTRICTION FRAGMENT LENGTH POLYMORPHISMS by selective PCR amplification of restriction fragments derived from genomic DNA followed by electrophoretic analysis of the amplified restriction fragments.
• Tree numbers
  • E05.393.290.382
  • E05.393.620.500.324
• Qualifiers
  drug effects, radiation effects, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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