Recombinant LC3B Antibody / N-Terminal

• Description:

  This antibody reacts to human LC3B (Autophagy-related protein LC3 B; Microtubule-associated proteins 1A/1B light chain 3B) . It may also react to mouse or rat LC3B, as predicted by immunogen homology.

• Specifications:

  Immunohistochemistry (FFPE) : 1:100-1:200, Immunocytochemistry: 1:100-1:200, Western Blot: 1:100-1:200

• UniProt:

  Q9GZQ8

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  A peptide corresponding to the N-terminus of human LC3B was used as the immunogen for the recombinant LC3B antibody.

• Clonality:

  Recombinant Monoclonal

• Isotype:

  IgG

• Clone:

  RM293

• Type:

  Recombinant

• Applications:

  WB, IHC, ICC

• Purity:

  Protein A purified from animal origin-free supernatant

• Format:

  Purified

• Buffer:

  Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

• Reconstitution:

  Store the recombinant LC3B antibody at -20oC.

• Limitations:

  This recombinant LC3B antibody is available for research use only.

• Storage Conditions:

  Store the recombinant LC3B antibody at -20°C.

• Formulation:

  Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

• Applications Notes:

  The stated application concentrations are suggested starting points. Titration of the recombinant LC3B antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9000-83-3

• Image Legend:

  ICC staining of human HeLa cells untreated or treated with chloroquine (CHQ), with recombinant LC3B antibody (red) at 1:200 dilution. Actin filaments have been labeled with fluorescein phalloidin (green), and nucleus labeled with DAPI (blue) .