LC3B Antibody

• Description :

  Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole) . MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. These proteins are involved in formation of autophagosomal vacuoles (autophagosomes) . MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. MAP1LC3b is one of the light chain subunits and can associate with either MAP1A or MAP1B. The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.

• Specifications :

  Western blot: 1:1000, Immunofluorescence: 1:25

• UniProt :

  Q9GZQ8

• Host :

  Rabbit

• Reactivity :

  Human, Rat

• Immunogen :

  A portion of amino acids 1-30 from the human protein was used as the immunogen for this LC3B antibody.

• Clonality :

  Polyclonal

• Isotype :

  Ig

• Applications :

  IF, WB, IHC, ELISA

• Purity :

  Purified

• Format :

  Purified

• Buffer :

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution :

  Aliquot the LC3B antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations :

  This LC3B antibody is available for research use only.

• Storage Conditions :

  Aliquot the LC3B antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation :

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes :

  Titration of the LC3B antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• Prediction Reactivity :

  Bovine

• CAS Number :

  9007-83-4

• Image Legend :

  Western blot analysis of lysate from human HepG2 and mouse NIH3T3 cell line, untreated or treated with chloroquine (50uM) using LC3B antibody at 1:1000.