EasyStep Human IGFBP3 (Insulin Like Growth Factor Binding Protein 3) ELISA Kit

EasyStep Human IGFBP3 (Insulin Like Growth Factor Binding Protein 3) ELISA Kit

• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IGFBP3, and the Human IGFBP3 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human IGFBP3. After TMB substrate solution is added, only those wells that contain Human IGFBP3 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IGFBP3 in the samples is then determined by comparing the OD of the samples to the standard curve.
• CAS Number: 7732-18-5
• Gene Aliases: BP53; IBP3; Growth Hormone-Dependent Binding Protein; Acid Stable Subunit Of The 140 K IGF Complex
• Field of Research: Metabolic pathway; Tumor immunity.
• Assay Principle: Sandwich ELISA
• Assay Performance Time: 1.5h
• Standard: 5000 pg/mL
• Sample Type: Serum, plasma
• Detection Range: 78.13-5000 pg/mL
• Sensitivity: 0.98 pg/mL