Antigen-Down ELISA Development Kit

• Description:

  This kit provides seven specially formulated ELISA solutions and a template for the initial development and optimization of antigen-down immunoassays by both novice and experienced users. The kit contains enough reagent for ten 96-well ELISA plates.

• Assay Principle:

  1. Prepare samples., 2. Create controls with CCCP., 3. Dilute 10X Assay Buffer 1:10 with diH2O., 4. Reconstitute MitoPT TMRE with DMSO., 5. Dilute MitoPT TMRE with 1X Assay Buffer., 6. Add MitoPT TMRE to each sample., 7. Incubate 15-30 minutes., 8. Wash cells: add 1X Assay Buffer and spin cells., 9. Remove supernatant and resuspend cells for analysis., 10. Anazlyze with a fluorescence microscope, fluorescence plate reader, or flow cytometer. MitoPT TMRE excites at 549 nm and emits at 574 nm (orange).

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  4°C

• CAS Number:

  7732-18-5