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Antigen-Down ELISA Development Kit

CAT: 0246-9101Size: 1 KitDry Ice: NoHazardous: No
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CAT#:0246-9101Size:1 Kit
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This kit provides seven specially formulated ELISA solutions and a template for the initial development and optimization of antigen-down immunoassays by both novice and experienced users. The kit contains enough reagent for ten 96-well ELISA plates.
CAS Number
7732-18-5
Assay Principle
1. Prepare samples., 2. Create controls with CCCP., 3. Dilute 10X Assay Buffer 1:10 with diH2O., 4. Reconstitute MitoPT TMRE with DMSO., 5. Dilute MitoPT TMRE with 1X Assay Buffer., 6. Add MitoPT TMRE to each sample., 7. Incubate 15-30 minutes., 8. Wash cells: add 1X Assay Buffer and spin cells., 9. Remove supernatant and resuspend cells for analysis., 10. Anazlyze with a fluorescence microscope, fluorescence plate reader, or flow cytometer. MitoPT TMRE excites at 549 nm and emits at 574 nm (orange).
Shipping Conditions
Blue Ice
Storage Conditions
4°C

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