Antigen Coating Buffer, 5X

Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Dry Ice Shipment: No

Alternative Products

Cat	Name
MBS258387-01	Antigen Coating Buffer, 5X
MBS258387-03	Antigen Coating Buffer, 5X
MBS258387-04	Antigen Coating Buffer, 5X
MBS258387-02	Antigen Coating Buffer, 5X
IBS-BC012	Coating Buffer
RM01756	Coating Buffer
abx098970-100l	Coating Buffer
abx098970-200l	Coating Buffer
MBS2090463-1Bottle	Coating Buffer
DS0236-100ML	Coating Buffer

CAT:

246-6247

Size:

100 mL

Price:

Ask

For price, please contact [email protected].

Antigen Coating Buffer, 5X

Description: Antigen Coating Buffer maximizes the adsorption of antigens onto polystyrene plates and stabilizes their 3-D structure to enhance specific signal, reduce reagent consumption, and extend the shelf-life of coated plates.
Target Antigen: Poly caspases
Assay Principle: 1. Dilute Antigen Coating Buffer, 5X 1:5 using deionized water and mix for 15 minutes. As Antigen Coating Buffer is a 5X concentrate, crystalline precipitates may form in the bottle, especially when refrigerated. If this happens, gently warm the buffer until all crystals are dissolved. Do not let it boil., 2. Dilute your antigen into the coating buffer. Optimal coating concentration varies significantly from 0.2 µg/mL to 10 µg/mL., 3. Let the solution stir 10 – 15 minutes and pipette onto the plate. Optimal coating volume generally ranges from 50 – 200 µL per well. ICT recommends coating antibodies onto Immulon II HB plates., 4. Once added to the plate, incubate the coating solution from 8 – 24 hours at room temperature protected from light. Minimize evaporation by individually covering each plate with a plate sealing cover, wrapping a stack in plastic wrap, or placing plates in a humidified storage box and covering. , 5. After incubation, dump or aspirate the coating solution out of the wells., 6. Wash the plate twice with ICT’s ELISA Wash Buffer., 7. Aspirate and pipette one of ICT’s Blocking Buffers onto the plate at a higher volume than the coating solution (300 – 400 µL per well)., 8. Once added to the plate, incubate the block buffer from 8 – 24 hours at room temperature protected from light. Minimize evaporation by individually covering each plate with a plate sealing cover, wrapping a stack in plastic wrap, or placing plates in a humidified storage box and covering., 9. Aspirate the block buffer., 10. The assay can be run at this point, or the plate can be dried and packaged for later use., 11. Dry the plate by letting it sit in the fume hood covered with foil overnight, or dry in a drying chamber under vacuum from 3 – 6 hours at room temperature. When dry, seal the plate in an air-tight foil pouch with a desiccant and store at 2-8°C protected from light.
pH: pH: 7.8-8.2
Shipping Conditions: Ambient Temperature
Storage Conditions: Room Temperature
Shelf Life: 24 months from date of manufacture

Complementary Product Range

Our range of products, including Antigen Coating Buffer, 5X, falls within the same field of application and can be used across various research projects. These products are carefully selected for their high performance and reliability, supporting the diverse needs of modern laboratories. Whether for ongoing studies or future experiments, they are designed to help researchers achieve their scientific goals. Here are some related products that may be of interest for similar or future applications:

Each item in our collection, is tested for precision and quality, ensuring dependable solutions for your research. As we continue to incorporate the latest advancements, Gentaur ensures that our product range remains relevant and versatile, providing you with the tools needed for both current and future projects.

Find us on

Cat	Name
6247	Antigen Coating Buffer, 5X
6248	Antigen Coating Buffer, 5X
6249	Antigen Coating Buffer, 5X
644	Antibody Coating Buffer, 5X
646	Antibody Coating Buffer, 5X
645	Antibody Coating Buffer, 5X
641	ELISA Blocking Buffer
AR-6544-05	Citrate Buffer, 10X
AR-6545-05	EDTA Buffer, 10X
AR-6565-05	Phosphate Buffer, 10X