Green Fluorescent FAM-FLICACaspase-8 (LETD) Assay Kit

• Description:

  This in vitro assay employs the fluorescent inhibitor probe FAM-LETD-FMK to label active caspase-8 enzyme in living cells. Analyze samples using fluorescence microscopy, a fluorescence plate reader, or flow cytometry.

• Target Antigen:

  Caspase-10

• Assay Principle:

  1. Prepare samples., 2. Create controls with CCCP., 3. Dilute 10X Assay Buffer 1:10 with diH2O., 4. Reconstitute MitoPT JC-1 with DMSO., 5. Dilute MitoPT JC-1 with 1X Assay Buffer., 6. Add MitoPT JC-1 to each sample., 7. Incubate 15-30 minutes., 8. Wash cells: add 1X Assay Buffer and spin cells (twice)., 9. Analyze with a fluorescence microscope, fluorescence plate reader, or flow cytometer. MitoPT JC-1 excites at 488 nm. Aggregated MitoPT JC-1 (orange) emits at 590 nm, monomeric MitoPT JC-1 (green) emits at 527 nm.

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  4°C