Fixative

• Description:

  ICT’s Fixative is a formaldehyde solution designed to cross-link intracellular components. It will not interfere with the fluorescence labels used on our fluorescent detection probes like FLICA, Magic Red, and FLISP.

• Assay Principle:

  1. Add PI to the cell sample media at 0.5% v/v. For example, add 1.5 µL PI to 300 µL of cells. 2. Incubate 5-10 minutes at room temperature., 3. Visualize with a fluorescence microscope with excitation at 488 nm and emission at 635 nm., 4. Alternatively, cells can be analyzed with a flow cytometer using a blue laser at 488 nm or a green laser at 525 nm. Read fluorescent output in FL-2 or FL-3., 5. When bound to DNA, the maximum absorption is 535 nm and the maximum emission is 617.

• Concentration:

  250 µg/mL

• pH:

  pH: 4.5 + 1.0

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  4°C