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Gellan Gum

CAT: 0644-CN-G045-100GMSize: 100 gDry Ice: NoHazardous: No
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CAT#:0644-CN-G045-100GMSize:100 g
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Gellan gum, an anionic polysaccharide, is employed as a replacement for agar in various clinical bacteriological and plant tissue culture media. Its structural composition comprises a repeating tetrasaccharide unit, including two D-glucose residues, one L-rhamnose residue, and one D-glucuronic acid residue.
It is an excellent alternative for nutrient media in plant tissue culture, yielding a clearer and more transparent gel than agar. Additionally, it requires only half the concentration of agar typically used in tissue culture media.
  • Plant Tissue Culture Grade → Suitable for preparation of solid or semi-solid media used in in vitro plant culture. Effective Gelling Agent → Forms a firm gel matrix when incorporated into suitable aqueous culture media.
  • Effective Gelling Agent → Forms a firm gel matrix when incorporated into suitable aqueous culture media.
  • Clear Gel Formation → Produces a relatively clear culture medium, facilitating observation of plant tissues and roots.
  • Consistent Gel Matrix → Supports reproducible preparation of solid culture media for laboratory plant culture.
  • Micropropagation Applications → Suitable for plant propagation and regeneration workflows.
  • Plant Biotechnology → Useful for tissue culture, plant regeneration and other in vitro plant research applications.
  • 100 g Format → Convenient pack size for routine plant tissue culture and laboratory research.
CAS Number
71010-52-1
Synonyms
Phytagel, Gelzan
HS Code
1302.39
Applications
Gellan Gum is used in doses ranging between 0.2% (2g/L) to 0.4% (4g/L) (w/v) to solidify plant tissue culture media.
Gellan Gum is used as a microbial solid culturing medium.

  • Plant Tissue Culture → Used as a gelling component in solid or semi-solid plant culture media.
  • Micropropagation → Supports culture media used for plant multiplication and regeneration.
  • Plant Regeneration → Provides a stable matrix for cultured plant tissues and explants.
  • In Vitro Plant Culture → Suitable for controlled cultivation of plant tissues, shoots and roots.
  • Plant Biotechnology → Useful in research involving plant propagation, tissue culture and regeneration.
  • Culture Medium Preparation → Can be incorporated into appropriate basal media such as DKW, MS and other plant tissue culture formulations.
Assay Protocol
Follow the steps below to prepare 1 liter of tissue culture media using PCT’s Gellan Gum :
  • Take a beaker and add 800 ml of distilled water to it.
  • To the water add PCT’s MS Media (or alternative media) 4.54 grams, 30 grams of sugar, and PPM™ (generally 1-2 ml/L).
  • Make up the volume of the media to 1000ml.
  • Adjust the pH of the media between 5.4-5.8.
  • Add 3-4 g/L of PCT’s Gellan Gum to the media and keep stirring.
  • Autoclave the medium for 20 minutes at 15 psi and 121°C.
Your media is ready!
Capacity
Available in different weights of 100 gm, 500 gm, 1 Kg, and 5 Kg
Format
Cylindrical container
Material
Powder
Color
Off White
Features & Benefits
  • Easy to use
  • Acts as a solid support for the growth and development of explants
  • Allows the absorption of nutrients by the plant cells
  • The transparency of the gel allows one to clearly see the development of roots or contamination from the start.
  • Cost-effective
  • Solidifying agents play a crucial role in tissue culture media by providing the necessary support to hold cells, tissues, or explants in place and ensure they receive optimal nutrients for growth and development.
Storage Temperature
20-22 °C
Shelf Life
See expiry date on the bottle
Strength
>900 g/cm^2
FAQ
What is gellan gum used for in plant tissue culture? → Gellan gum is used as a gelling agent for solid or semi-solid plant tissue culture media, providing a stable matrix for plant explants, shoots and roots. Can gellan gum replace agar in plant tissue culture? Yes. Gellan gum can be used as an alternative gelling agent to agar in suitable plant tissue culture formulations. The optimal concentration should be determined according to the specific medium and plant culture protocol. Can gellan gum be used with MS medium? → Yes. Gellan gum can be incorporated into suitable Murashige & Skoog (MS) and other plant tissue culture media to produce solid or semi-solid culture media. Can gellan gum be used with DKW medium? → Yes. Gellan gum can be used as a gelling component in appropriate DKW plant tissue culture formulations. Is gellan gum suitable for micropropagation? → Yes. Gellan gum can be used in culture media for plant micropropagation, regeneration and in vitro cultivation. What is the size of this product? → This product is supplied as 100 g, under Gentaur CAT#: 0644-CN-G045-100GM.
Certificate of Analysis
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Product MSDS
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