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Chaps Lysis and Immunoprecipitation Buffer

CAT: 0605-CIB-1-8.0-60Size: 60 mLDry Ice: NoHazardous: No
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CAT#:0605-CIB-1-8.0-60Size:60 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
CHAPS Buffer Strong cell lysis and protein extraction capacity while preserving protein conformation and protein-protein interaction for co-immunoprecipitation (Co-IP) analysis. Can be used with tissues (see product citation 1 below) as well as culture cells. Effective as a stand alone reagent for cell lysis, protein extraction and subsequent Western blot in addition to immunoprecipitation. Contains a non-amine buffer (HEPES) enabling cross-linking with NHS-ester derivatives. Ingredients:  0.5% CHAPS, HEPES, NaClCompatible with Bradford, Lowry and BCA protein assays. 60 mL volume:  Suitable for two hundred 10 cm culture plates, or six hundred wells in 6-well dishes.  120 mL volume for four hundred 10 cm culture plates or 1200 wells in 6-well dishes.
pH
pH 8.0
Storage Conditions
4°C