Bis-Tris

• Description :

  Bis-Tris is an amine buffer suitable for protein and nucleic acid systems with a pH buffer range of 5.8-7.2. Bis-Tris can also be mixed with HEPES and cacodylic acid buffer to create a pH 8 environment and monitor fluorescence emission intensity at 305 nm. Gels formulated with Bis-Tris also avoid protein degradation in samples prepared at higher pH (pH 8.5) [1][2][3].

• UNSPSC :

  12352200

• Hazard Statement :

  H318

• Target :

  Biochemical Assay Reagents

• Type :

  Biochemical Assay Reagents

• Related Pathways :

  Others

• Field of Research :

  Others

• Assay Protocol :

  https://www.medchemexpress.com/bis-tris.html

• Purity :

  99.91

• Solubility :

  H2O : 200 mg/mL (ultrasonic)

• Smiles :

  OCC(CO)(N(CCO)CCO)CO

• Molecular Formula :

  C8H19NO5

• Molecular Weight :

  209.24

• Precautions :

  H318

• References & Citations :

  [1]Oh, Soon Jin, et al. Structure and phosphodiesterase activity of Bis-Tris coordinated lanthanide (III) complexes.|[2]Hachmann JP, et al. Models of protein modification in Tris-glycine and neutral pH Bis-Tris gels during electrophoresis: effect of gel pH. Anal Biochem. 2005 Jul 15;342 (2) :237-45.|[3]Williams AS, et al. Domain 4 of the anthrax protective antigen maintains structure and binding to the host receptor CMG2 at low pH. Protein Sci. 2009 Nov;18 (11) :2277-86.

• Shipping Conditions :

  Room Temperature

• Storage Conditions :

  Store at room temperature, keep dry and cool

• Scientific Category :

  Biochemical Assay Reagents

• Clinical Information :

  No Development Reported

• CAS Number :

  [6976-37-0]