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Bis-Tris

CAT: 0804-HY-D0856-01Size: 25 gDry Ice: NoHazardous: No
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CAT#:0804-HY-D0856-01Size:25 g
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Bis-Tris is an amine buffer suitable for protein and nucleic acid systems with a pH buffer range of 5.8-7.2. Bis-Tris can also be mixed with HEPES and cacodylic acid buffer to create a pH 8 environment and monitor fluorescence emission intensity at 305 nm. Gels formulated with Bis-Tris also avoid protein degradation in samples prepared at higher pH (pH 8.5) [1][2][3].
CAS Number
6976-37-0
UNSPSC
12352200
Hazard Statement
H318
Target
Biochemical Assay Reagents
Type
Biochemical Assay Reagents
Related Pathways
Others
Field of Research
Others
Assay Protocol
https://www.medchemexpress.com/bis-tris.html
Purity
99.91
Solubility
H2O : 200 mg/mL (ultrasonic)
Smiles
OCC(CO)(N(CCO)CCO)CO
Molecular Formula
C8H19NO5
Molecular Weight
209.24
Precautions
H318
References & Citations
[1]Oh, Soon Jin, et al. Structure and phosphodiesterase activity of Bis-Tris coordinated lanthanide (III) complexes.|[2]Hachmann JP, et al. Models of protein modification in Tris-glycine and neutral pH Bis-Tris gels during electrophoresis: effect of gel pH. Anal Biochem. 2005 Jul 15;342 (2) :237-45.|[3]Williams AS, et al. Domain 4 of the anthrax protective antigen maintains structure and binding to the host receptor CMG2 at low pH. Protein Sci. 2009 Nov;18 (11) :2277-86.
Shipping Conditions
Room Temperature
Storage Conditions
Store at room temperature, keep dry and cool
Scientific Category
Biochemical Assay Reagents
Clinical Information
No Development Reported

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