Anti-Granzyme B (NK/T-Cell Lymphoma Marker) (GZMB/2403), CF640R conjugate

• Description:

  Granzyme B is a member of the granule serine protease family stored specifically in NK cells or cytotoxic T cells. Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. Granzyme B is crucial for the rapid induction of target cell apoptosis by CTLs in the cell-mediated immune response. Granzyme B is useful as a marker in the identification of NK/T-cell lymphomas. High percentages of cytotoxic T-cells have been shown to be an unfavorable prognostic indicator in Hodgkin's Disease.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms:

  Cathepsin G-like 1; CCPI; CGL1; CSPB; CTLA-1; CTSGL1; Cytotoxic serine protease B; Cytotoxic T lymphocyte associated serine esterase 1; Cytotoxic T-lymphocyte proteinase 2; Fragmentin-2; Granzyme B; Granzyme-2; GRB; Gzmb; Human lymphocyte protein (Hlp); Lymphocyte protease; SECT; T-cell serine protease 1-3E

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  GZMB

• Gene ID:

  3002

• NCBI Gene ID:

  1051

• UniProt:

  P10144

• Cellular Locus:

  Secreted (extracellular)|Vesicular

• Host:

  Mouse

• Species Reactivity:

  Human

• Immunogen:

  Recombinant fragment of human GZMB protein (around aa 73-187) (exact sequence is proprietary)

• Target Antigen:

  Granzyme B

• Clonality:

  Monoclonal

• Isotype:

  IgG2b

• Clone:

  GZMB/2403

• Conjugation:

  CF640R

• Disease:

  Tumor

• Source:

  Animal

• Field of Research:

  Immunology

• Positive Control:

  A431 cells. Tonsil, Spleen or Hodgkin's Lymphoma

• Concentration:

  0.1 mg/mL

• Buffer:

  PBS, 0.1% BSA, 0.05% azide

• Molecular Weight:

  29-32 kDa

• Additionnal Information:

  For coating for ELISA, order Ab without BSA|Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Optimal dilution and staining procedure for a specific application should be determined by user|Recommended starting concentrations for titration are 1-2 ug/mL for most applications, or 1 ug/million cells/100 uL for flow cytometry

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years

• CAS Number:

  9007-83-4