Anti-TCF4 (Transcription Factor 4) (TCF4/1705), CF640R conjugate

• Description:

  This antibody recognizes a protein of 71 kDa, identified as Transcription Factor 4 (TCF4). It is a basic helix-loop-helix transcription factor. The protein recognizes an Ephrussi-box (E-box) binding site (CANNTG) - a motif first identified in immunoglobulin enhancers. This gene is broadly expressed, and may play an important role in nervous system development. Defects in this gene are a cause of Pitt-Hopkins syndrome._x000D_ _x000D_ Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors._x000D_ _x000D_

• Synonyms:

  bHLHb19; Class B basic helix-loop-helix protein 19; Immunoglobulin transcription factor 2; ITF-2; PTHS; SEF2; SL3-3 enhancer factor 2; TCF4; Transcription factor 4

• CAS Number:

  9007-83-4

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  TCF4

• Gene ID:

  6925

• NCBI Gene ID:

  605153

• UniProt:

  P15884

• Cellular Locus:

  Nucleus

• Host:

  Mouse

• Species Reactivity:

  Human

• Immunogen:

  Recombinant human TCF4 protein fragment (around aa 365-671) (exact sequence is proprietary)

• Target Antigen:

  TCF4

• Clonality:

  Monoclonal

• Isotype:

  IgG2a κ

• Clone:

  TCF4/1705

• Conjugation:

  CF640R

• Source:

  Animal

• Applications:

  WB (verified)

• Validated Applications:

  WB

• Field of Research:

  Developmental biology, Transcription factors

• Positive Control:

  HeLa or HepG2 cells. Bladder

• Concentration:

  0.1 mg/mL

• Buffer:

  PBS, 0.1% BSA, 0.05% azide

• Molecular Weight:

  71 kDa

• Additionnal Information:

  ELISA: Use Ab at 2-4 ug/mL for coating; Order Ab without BSA; Western blot 1-2 ug/mL; Optimal dilution for a specific application should be determined.|Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years