Anti-CLEC9A / DNGR1 (Dendritic Cell Marker)(8F9), CF740 conjugate

• Description: This MAb recognizes a protein of 27 kDa, identified as CLEC9A (C-type lectin domain family 9 member A). Its epitopes maps in aa 50-110. The C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily consists of a variety of proteins that share a common protein fold and have diverse functions, including cell-cell signaling, cell adhesion, glycoprotein turnover and immune responses. CLEC-9A, also known as DNGR1 (dendritic cell natural killer lectin group receptor 1), is a 241 amino acid single-pass type II membrane protein that contains one C-type lectin domain and belongs to the CTL/CTLD superfamily. Expressed in myeloid lineage cells, brain, spleen and thymus, CLEC-9A is a group V C-type lectin- like receptor (CTLR) that acts as an activation receptor. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
• Synonyms: C-type lectin domain family 9 member A (CLEC9A); DNGR1
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: CLEC9A
• Gene ID: 283420
• NCBI Gene ID: 531189
• UniProt: Q6UXN8
• Cellular Locus: Plasma membrane
• Host: Mouse
• Species Reactivity: Human
• Immunogen: RBL-2H3 cells expressing human DNGR-1 fused to an HA epitope
• Target Antigen: CLEC9A | DNGR1
• Clonality: Monoclonal
• Isotype: IgG2a κ
• Clone: 8F9
• Conjugation: CF740
• Source: Animal
• Applications: Flow, surface (published) | IF (published)
• Validated Applications: FC, IF
• Positive Control: MCF-7 cells (FACS/IF).
• Concentration: 0.1 mg/mL
• Buffer: PBS, 0.1% rBSA, 0.05% azide
• Molecular Weight: 27 kDa
• Additionnal Information: ELISA: For coating, order antibody without BSA; Flow cytometry: 0.5-1 ug/million cells; Immunofluorescence: 0.5-1 ug/mL; Optimal dilution for a specific application should be determined.|Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody
• References & Citations: Note: References for this clone sold by other suppliers may be listed for expected applications. J Clin Invest (2008) 118(6):2098-2110. (IF, Flow) Clinical Translational Immunol (2016) 5:e57. (Flow)
• Shipping Conditions: Room temperature
• Storage Conditions: 4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years