Anti-CD81 / TAPA-1 (rC81/3442), CF740 conjugate

• Description:

  This antibody recognizes a protein of 26 kDa, identified as CD81 (Workshop VI; Code CD81.1)). CD81 has a very broad cellular distribution, being expressed on T- and B-lymphocytes, NK cells, thymocytes, eosinophils, fibroblasts, epithelial and endothelial cells. Neutrophils, erythrocytes and platelets are negative, while monocytes are variably positive. CD81 is a member of a family of tetraspanin transmembrane proteins, including CD9, CD37, CD53, CD63, and CD82. It associates with CD19, CD21, Leu 13, and integrins on cell membrane and is involved in signal transduction in B lymphocyte development and cell adhesion. CD81 also acts as a receptor for the envelope protein E2 of chronic hepatitis C virus. Antibodies to CD81 have anti-proliferative effects on different lymphoid cell lines, particularly those derived from large cell lymphomas._x000D_ _x000D_ Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors._x000D_ _x000D_

• Synonyms:

  CD81; Target of the antiproliferative antibody 1 (TAPA-1); Tetraspanin-28; Tspan-28

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  CD81

• Gene ID:

  975

• NCBI Gene ID:

  54457

• UniProt:

  P60033

• Cellular Locus:

  Exosomes/EVs|Plasma membrane

• Host:

  Mouse

• Species Reactivity:

  Human, Rat

• Immunogen:

  Recombinant full-length human CD81 protein

• Target Antigen:

  CD81 | TAPA-1

• Clonality:

  Recombinant Monoclonal

• Isotype:

  IgG1 κ

• Clone:

  rC81/3442

• Conjugation:

  CF740

• Source:

  Animal

• Applications:

  IF | Exosome staining (verified) | Flow, surface (verified) | IHC, FFPE (verified)

• Validated Applications:

  IF, FC, IHC, FFPE

• Field of Research:

  Exosomes/EVs, Immunology

• Positive Control:

  Ramos, Daudi, Raji, U266, HUT-78, HUT-102, Jurkat, HL-60, KG1a, A293, A431, MG63, MCF-7, and human lymphocytes. Tonsil or liver tissue.

• Concentration:

  0.1 mg/mL

• Buffer:

  PBS, 0.1% rBSA, 0.05% azide

• Molecular Weight:

  26 kDa

• Additionnal Information:

  Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes|Immunohistology (formalin): 2-4 ug/mL for 30 minutes at RT|For functional studies, order Ab without azide|Western blotting 1-2 ug/mL|Immunofluorescence 0.5-1 ug/mL|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years

• CAS Number:

  9007-83-4