Anti-Cyclin A1(XLA1-3), CF740 conjugate

• Description:

  The critical role that the family of regulatory proteins known as cyclins play in eukaryotic cell cycle regulation is well established. The best-characterized cyclin complex is the mitotic cyclin B/Cdc2 p34 kinase, the active component of maturing promoting factor. Cyclin A accumulates prior to cyclin B in the cell cycle, appears to be involved in control of S phase and has been shown to associate with cyclin-dependent kinase-2 (Cdk2). In addition, cyclin A has been implicated in cell transformation and is found in complexes with E1A, transcription factors DRTF1 and E2F, and retinoblastoma protein p110. A second form of cyclin A, named cyclin A1 because of its high sequence homology to Xenopus cyclin A1, is most highly expressed in germ cells. It has been proposed that cyclin A1 can associate with Cdk2, p39 and Cdc2 p34. The antibody detects Xenopus cyclin A in blots. Cyclin A1 is the embryonic cyclin which is present in Xenopus eggs. This MAb only detects Xenopus Cyclin A1. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms:

  CCN1; CCNA; CCNA2; Cyclin A1

• CAS Number:

  9007-83-4

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  ccna1

• Gene ID:

  Not Known

• NCBI Gene ID:

  4267

• UniProt:

  P18606

• Cellular Locus:

  Nucleus

• Host:

  Mouse

• Species Reactivity:

  Xenopus

• Immunogen:

  Recombinant full-length Xenopus laevis Cyclin A1 protein

• Target Antigen:

  Cyclin A1

• Clonality:

  Monoclonal

• Isotype:

  IgG1 κ

• Clone:

  XLA1-3

• Conjugation:

  CF740

• Source:

  Animal

• Applications:

  WB (verified)

• Validated Applications:

  WB

• Positive Control:

  Very high levels in testis. Also found in myeloid leukemia cell lines. Xenopus laevis tissue lysate.

• Concentration:

  0.1 mg/mL

• Buffer:

  PBS, 0.1% rBSA, 0.05% azide

• Molecular Weight:

  65 kDa

• Additionnal Information:

  Western blot 1-2 ug/mL; Optimal dilution for a specific application should be determined.

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years