Anti-Golgi Complex (Marker for Human Cells) (GLG1/2829R), CF740 conjugate
CAT:
37-BNC742829-100
Size:
100 µL
Price:
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- Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
- Dry Ice Shipment: No
Anti-Golgi Complex (Marker for Human Cells) (GLG1/2829R), CF740 conjugate
- Description: This MAb recognizes an antigen associated with the Golgi complex in human cells only. It can be used to stain the Golgi complex in cell or tissue preparations and can be used as a Golgi marker in subcellular fractions. It produces a diffuse staining pattern of the Golgi zone in normal and malignant cells. This MAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. The Golgi apparatus is an organelle present in all eukaryotic cells that forms a part of the endomembrane system. The primary function of the Golgi apparatus is to process and package macromolecules synthesized by the cell for exocytosis or use within the cell. The Golgi is made up of a stack of flattened, membrane-bound sacs known as cisternae, with three functional regions: the cis face, medial region and trans face. Each region consists of various enzymes that selectively modify the macromolecules passing though them, depending on where they are destined to reside. Several spherical vesicles that have budded off of the Golgi are present surrounding the main cisternae._x000D_ _x000D_ Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors._x000D_ _x000D_
- Synonyms: Golgi Complex; Golgi apparatus; Golgi zone
- CAS Number: 9007-83-4
- UNSPSC: 41116161
- UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
- Gene Name: Not Known
- Gene ID: Not Known
- NCBI Gene ID: Not Known
- UniProt: Not Known
- Cellular Locus: Golgi apparatus
- Host: Rabbit
- Species Reactivity: Human
- Immunogen: SU-DHL-1 large cell lymphoma cells.
- Target Antigen: Golgi Complex
- Clonality: Recombinant Monoclonal
- Isotype: IgG
- Clone: GLG1/2829R
- Conjugation: CF740
- Source: Animal
- Applications: IHC, FFPE (verified)
- Validated Applications: IHC, FFPE
- Field of Research: Organelle markers
- Positive Control: HepG2, A431 or HeLa cells. Placenta, Tonsil, Testis and Ovary.
- Concentration: 0.1 mg/mL
- Buffer: PBS, 0.1% rBSA, 0.05% azide
- Molecular Weight: Not Known
- Additionnal Information: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Does not react with mouse or rat|Immunohistology (formalin): 0.5-1 ug/mL for 30 minutes at RT|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes|Optimal dilution for a specific application should be determined by user
- Shipping Conditions: Room temperature
- Storage Conditions: 4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.
- Shelf Life: 2 years