Anti-Replication Protein A2 (RPA2)(RPA2/3140R), CF740 conjugate

• Description:

  Recognizes a protein of 34 kDa, identified as a subunit of Replication Protein A (RPA) (also known as human single-stranded DNA binding protein, or HSSB). RPA from human cells is a stable heterotrimer of 70 kDa, 32-34 kDa, and 11-14 kDa subunits (RPA70, RPA32, and RPA14 respectively). It is involved in DNA replication, repair, and recombination. RPA is required for the SV40 large tumor antigen-catalyzed unwinding of SV40 DNA and stimulates DNA polymerase (pol) alpha and delta. RPA34 is phosphorylated at the G1/S boundary of the cell cycle or upon exposure of cells to DNA damage-inducing agents including ionizing and UV radiation. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms:

  p32; p34; REPA2; Replication protein A 32kDa subunit; Replication protein A 34kDa subunit; Replication protein A2; RF-A protein 2; Rf-A2; RFA; RP-A p32; RP-A p34; RP21C; RPA32; RPA34; RpLP1; RpP2

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  RPA2

• Gene ID:

  6118

• NCBI Gene ID:

  703070; 79411

• UniProt:

  P15927

• Cellular Locus:

  Nucleus

• Host:

  Rabbit

• Species Reactivity:

  Human

• Immunogen:

  Recombinant human full-length Replication protein A2 (RPA2) protein

• Target Antigen:

  Replication Protein A2

• Clonality:

  Recombinant Monoclonal

• Isotype:

  IgG

• Clone:

  RPA2/3140R

• Conjugation:

  CF740

• Source:

  Animal

• Applications:

  WB (verified)

• Validated Applications:

  WB

• Positive Control:

  MCF-7, HeLa or HepG2 cell lysates (WB). Human tonsil (IHC).

• Concentration:

  0.1 mg/mL

• Buffer:

  PBS, 0.1% rBSA, 0.05% azide

• Molecular Weight:

  32-34 kDa

• Additionnal Information:

  Western blot 1-2 ug/mL; Optimal dilution for a specific application should be determined.

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years

• CAS Number:

  9007-83-4