Human ENPP-2 / Autotaxin Protein, His Tag (active enzyme)

• Background:

  Autotaxin also known as ectonucleotide pyrophosphatase / phosphodiesterase family member 2 (E-NPP 2 or ENPP2), Extracellular lysophospholipase D (LysoPLD), Autotaxin (ATX or ATX-X), which belongs to the nucleotide pyrophosphatase / phosphodiesterase family. ENPP2 contains two SMB (somatomedin-B) domains. ENPP2 hydrolyzes lysophospholipids to produce lysophosphatidic acid (LPA) in extracellular fluids. ENPP2 can act on sphingosylphosphphorylcholine producing sphingosine-1-phosphate, a modulator of cell motility. ENPP2 stimulates migration of melanoma cells, probably via a pertussis toxin-sensitive G protein.

• Description:

  Human ENPP-2, His Tag (EN2-H5241) is expressed from human 293 cells (HEK293) . It contains AA Asp 49 - Ile 863 (Accession # NP_001035181) .

• Specifications:

  This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 94.5 kDa. The protein migrates as 90-100 kDa under reducing (R) condition (SDS-PAGE) .

• Accession Number:

  NP_001035181

• Expression Region:

  Asp 49 - Ile 863

• Host:

  HEK293

• Target:

  ENPP-2

• Conjugation:

  Unconjugated

• Tag:

  N-6xHis

• Source:

  Human

• Stability:

  ● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

• Endotoxin:

  1.0 EU per μg

• Purity:

  95%

• Format:

  Powder

• Buffer:

  50 mM Tris, 150 mM NaCl, pH7.5

• Additives:

  Trehalose

• Molecular Weight:

  94.5 kDa

• Shipping Conditions:

  RT

• Storage Conditions:

  -20°C

• Package Size:

  250ug*1

• Species:

  Human