Anti-Perforin-1 (Pore Forming Protein) (Apoptosis Marker) (PRF1/2468)

Anti-Perforin-1 (Pore Forming Protein) (Apoptosis Marker) (PRF1/2468)

• Description: Perforin is a pore-forming protein that leads to osmotic lysis of the target cells and subsequently enables granzymes to enter the target cells and activate apoptosis. Perforin has structural and functional similarities to complement component 9 (C9). Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. It is one of the main cytolytic proteins of cytolytic granules, and is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in this gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood. The expression of perforin is reportedly upregulated in activated CD8 T-cells, natural killer cells and some CD4 T-cells.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
• Synonyms: Cytolysin; FLH2; HPLH2; Lymphocyte pore-forming protein; PRF1 (pore forming protein 1); Perforin-1; PFP; PGFL; PIGF; PIGF-2; PLGF
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: PRF1
• Gene ID: 5551
• NCBI Gene ID: 2200
• UniProt: P14222
• Cellular Locus: Secreted (extracellular)
• Host: Mouse
• Species Reactivity: Human
• Immunogen: Recombinant human Perforin-1 protein fragment (around aa 413-552) (exact sequence is proprietary)
• Target Antigen: Perforin-1
• Clonality: Monoclonal
• Isotype: IgG2b κ
• Clone: PRF1/2468
• Conjugation: Purified, with BSA
• Source: Animal
• Field of Research: Immunology
• Positive Control: HDLM-2 Cells. Spleen
• Concentration: 0.2 mg/mL
• Buffer: PBS, 0.05% BSA, 0.05% azide
• Molecular Weight: 75 kDa
• Additionnal Information: For coating for ELISA, order Ab without BSA|Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Optimal dilution and staining procedure for a specific application should be determined by user|Recommended starting concentrations for titration are 1-2 ug/mL for most applications, or 1 ug/million cells/100 uL for flow cytometry
• Shipping Conditions: Room temperature
• Storage Conditions: 4°C; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years