Anti-TIMP2 (Tissue Inhibitor of Metalloproteinase 2) (TIMP2/2488R)

• Description:

  This antibody recognizes a protein of 21 kDa, identified as tissue inhibitor of metalloproteinases-2 (TIMP-2). It is closely related to TIMP-1 and shows the highest binding affinity to both the latent (pro) and active forms of 72 kDa Type IV collagenase (also known as MMP-2 or gelatinase A). It also has affinity for the active form of 92 kDa Type IV collagenase (also known as MMP-9 or gelatinase B). TIMP-2 inhibits the proteolytic invasiveness of tumor cells and normal placental trophoblast cells.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms:

  Metalloproteinase inhibitor 2; TIMP metallopeptidase inhibitor 2; TIMP-2; TIMP2; Tissue Inhibitor of Metalloproteinase 2

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  TIMP2

• Gene ID:

  7077

• NCBI Gene ID:

  633514

• UniProt:

  P16035

• Cellular Locus:

  Cytoplasmic|Secreted (extracellular)

• Host:

  Rabbit

• Species Reactivity:

  Cow, Guinea pig, Human, Mouse, Rabbit, Rat, Zebrafish

• Immunogen:

  A synthetic peptide from the N-terminus of human TIMP2 protein (aa56-66) (DSGNDIYGNPI)

• Target Antigen:

  TIMP2

• Clonality:

  Recombinant Monoclonal

• Isotype:

  IgG

• Clone:

  TIMP2/2488R

• Conjugation:

  Purified, with BSA

• Source:

  Animal

• Applications:

  IHC, FFPE (verified)

• Validated Applications:

  IHC, FFPE

• Field of Research:

  Extracellular matrix, Proteases

• Positive Control:

  HeLa cells. Normal Placenta. Breast, Colon, Endometrial, Prostate or Ovarian Carcinoma.

• Concentration:

  0.2 mg/mL

• Buffer:

  PBS, 0.05% BSA, 0.05% azide

• Molecular Weight:

  21 kDa

• Additionnal Information:

  Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunohistology (formalin): 1-2 ug/mL for 30 minutes at RT|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes|Optimal dilution for a specific application should be determined by user

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years

• CAS Number:

  9007-83-4