Anti-MUC1 / CA15-3 / EMA / CD227 (Epithelial Marker) (MUC1/1887R)

• Description:

  This MAb reacts with MUC1, a large transmembrane glycoprotein expressed on the ductal surface of normal glandular epithelia. It is used as tracer agent in CA15.3 assays. The extracellular domain of MUC1 largely consists of a highly conserved, O-glycosylated 20 amino acids tandem repeat which can occur 30-100 times per molecule depending on the length of the allele involved. In the vast majority of human carcinomas this protein is up-regulated and poorly glycosylated and appears on the cell surface in a non-polarized fashion. The dominant epitope of this MAb involves both amino acids as well as sugar moieties. Its epitope is destroyed by desialylation i.e. treatment with Neuraminidase.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms:

  Breast carcinoma-associated antigen DF3; CA15-3; Carcinoma-associated mucin Episialin; Epithelial Membrane Antigen; H23AG; KL-6; MAM6; MUC-1; MUC-1/SEC; MUC-1/X; MUC1-alpha; MUC1-beta; MUC1-CT; MUC1-NT; MUC1/ZD; Mucin 1 cell surface associated; Mucin-1 subunit beta; Peanut-reactive urinary mucin; PEM; PEMT; Polymorphic epithelial mucin; PUM; Tumor-associated epithelial membrane antigen

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  MUC1

• Gene ID:

  4582

• NCBI Gene ID:

  89603

• UniProt:

  P15941

• Cellular Locus:

  Plasma membrane

• Host:

  Rabbit

• Species Reactivity:

  Human

• Immunogen:

  Recombinant human MUC1 protein

• Target Antigen:

  CA15-3 | CD227 | EMA | MUC1

• Clonality:

  Recombinant Monoclonal

• Isotype:

  IgG

• Clone:

  MUC1/1887R

• Conjugation:

  Purified, with BSA

• Disease:

  Tumor

• Source:

  Animal

• Applications:

  IHC, FFPE (verified) | WB (verified)

• Validated Applications:

  IHC, FFPE, WB

• Field of Research:

  Cancer, Mucins

• Positive Control:

  MCF-7 or MDA-231 cells. Breast, colon, ovarian, endometrial carcinoma.

• Concentration:

  0.2 mg/mL

• Buffer:

  PBS, 0.05% BSA, 0.05% azide

• Molecular Weight:

  265-400 kDa

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years

• CAS Number:

  9007-83-4