Anti-ACTH (Adrenocorticotrophic Hormone) (Pituitary Marker) (rCLIP/1407)

• Description:

  ACTH (same as Corticotropin) is a 39 amino acid active peptide produced by the anterior pituitary. This MAb is specific to CLIP (aa25-39 of ACTH); does not react with Synacthen (aa1-24 of ACTH). POMC (pro-opiomelanocortin or corticotropin-lipotropin) is a 267 amino acid polypeptide hormone precursor that goes through extensive, tissue-specific posttranslational processing by convertases. POMC is cleaved into ten hormone chains named NPP, ACTH, alpha-MSH (Melanocyte Stimulating Hormone), beta-MSH, gamma-MSH, CLIP (corticotropin-like intermediary peptide), Lipotropin-beta, Lipotropin-gamma, beta-endorphin and Met-enkephalin. ACTH is also produced by cells of immune system (T-cells, B-cells, and macrophages) in response to stimuli associated with stress. Anti-ACTH is a useful marker in classification of pituitary tumors and the study of pituitary disease. It reacts with ACTH-producing cells (corticotrophs).It also may react with other tumors (e.g. some small cell carcinomas of the lung) causing paraneoplastic syndromes by secreting ACTH. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms:

  Pro-opiomelanocortin (POMC); Adrenocorticotropin; Adrenocorticotropic hormone; Melanocyte Stimulating Hormone (MSH); Melanotropin; beta-Endorphin; Lipotropin; Met-Enkephalin; POC

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  POMC

• Gene ID:

  5443

• NCBI Gene ID:

  1897

• UniProt:

  P01189

• Cellular Locus:

  Secreted (extracellular)|Vesicular

• Host:

  Mouse

• Species Reactivity:

  Multiple species, Human, Mouse, Rat

• Immunogen:

  Synthetic peptide corresponding to (aa 162-176) of human ACTH (NGAEDESAEAFPLEF)

• Target Antigen:

  ACTH

• Clonality:

  Recombinant Monoclonal

• Isotype:

  IgG1

• Clone:

  rCLIP/1407

• Conjugation:

  Purified, with BSA

• Disease:

  Tumor

• Source:

  Animal

• Applications:

  IHC, FFPE (verified)

• Validated Applications:

  IHC, FFPE

• Field of Research:

  Cancer, Endocrinology

• Positive Control:

  Normal pituitary gland or pituitary tumor

• Concentration:

  0.2 mg/mL

• Buffer:

  PBS, 0.05% BSA, 0.05% azide

• Molecular Weight:

  ACTH is ~5 kDa, and the POMC precursor is ~30 kDa. The molecular weight of POMC depends upon isoform variation and post-translational modifications.

• Additionnal Information:

  Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunohistology (formalin): 0.5-1 ug/mL for 30 minutes at RT|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes|Optimal dilution for a specific application should be determined by user

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years

• CAS Number:

  9007-83-4