Anti-p63 (Squamous, Basal & Myoepithelial Cell Marker) (TP63/2427)

Anti-p63 (Squamous, Basal & Myoepithelial Cell Marker) (TP63/2427)

• Description: p63 is a homolog of the tumor suppressor p53. It is identified in basal cells in the epithelial layers of a variety of tissues, including epidermis, cervix, urothelium, breast and prostate. p63 was detected in nuclei of the basal epithelium in normal prostate glands; however, it was not expressed in malignant tumors of the prostate. As a result, p63 has been reported as a useful marker for differentiating benign from malignant lesions in the prostate, particularly when used in combination with markers of high molecular weight cytokeratins and the prostate-specific marker AMACR (P504S). p63 has also been shown to be a sensitive marker for lung squamous cell carcinomas (SqCC), with a sensitivity of ~90%. Specificity for lung SqCC, vs. lung adenocarcinoma (LADC), is approximately 80%. In breast tissue, p63 has been identified in myoepithelial cells of normal ducts.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
• Synonyms: Amplified in squamous cell carcinoma (AIS); Chronic ulcerative stomatitis protein (CUSP); EEC3; Keratinocyte transcription factor KET; LMS; NBP; p40; P51/P63; p53 like transcription factor; p53-related protein p63; RHS; SHFM4; TAp63alpha; TP53CP; TP53L; TP63; TP73; TP73L; Transformation-related protein 63; Trp53rp1; Trp6;3; Tumor protein 63; Tumor protein p53-like; tumor protein p73-like
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: TP63
• Gene ID: 8626
• NCBI Gene ID: 137569
• UniProt: Q9H3D4
• Cellular Locus: Nucleus
• Host: Mouse
• Species Reactivity: Human
• Immunogen: Recombinant full-length human p63 protein
• Target Antigen: p63
• Clonality: Monoclonal
• Isotype: IgG2b κ
• Clone: TP63/2427
• Conjugation: Purified, with BSA
• Disease: Tumor
• Source: Animal
• Applications: IHC, FFPE (verified)
• Validated Applications: IHC, FFPE
• Field of Research: Cancer, Cell cycle, Tumor suppressors
• Positive Control: HEK293 cells or Prostate Carcinoma or Lung or bladder squamous cell carcinoma
• Concentration: 0.2 mg/mL
• Buffer: PBS, 0.05% BSA, 0.05% azide
• Molecular Weight: 63 kDa
• Additionnal Information: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunohistology (formalin): 1-2 ug/mL for 30 minutes at RT|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes|Optimal dilution for a specific application should be determined by user
• Shipping Conditions: Room temperature
• Storage Conditions: 4°C; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years