Anti-STAT3 / Signal Transducer and Activator of Transcription 3 (PCRP-STAT3-2F12)

Anti-STAT3 / Signal Transducer and Activator of Transcription 3 (PCRP-STAT3-2F12)

• Description: The specificity of this monoclonal antibody to its intended target was validated by HuProt™ Array, containing more than 19,000, full-length human proteins. STAT3 is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein._x000D_ _x000D_ Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors._x000D_ _x000D_
• Synonyms: APRF; HIES; ADMIO; ADMIO1
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: STAT3
• Gene ID: 6774
• NCBI Gene ID: 463059
• UniProt: P40763
• Cellular Locus: Nucleus & cytoplasm
• Host: Mouse
• Species Reactivity: Human
• Immunogen: Recombinant full-length human STAT3 protein
• Target Antigen: STAT3
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone: PCRP-STAT3-2F12
• Conjugation: Purified, with BSA
• Source: Animal
• Field of Research: Immunology, Inflammation, Signal transduction, Transcription factors
• Positive Control: A431 and Raji cells. Kidney, Brain or Heart
• Concentration: 0.2 mg/mL
• Buffer: PBS, 0.05% BSA, 0.05% azide
• Molecular Weight: 88 kDa
• Additionnal Information: For coating for ELISA, order Ab without BSA|Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Optimal dilution and staining procedure for a specific application should be determined by user|Recommended starting concentrations for titration are 1-2 ug/mL for most applications, or 1 ug/million cells/100 uL for flow cytometry
• References & Citations: Zhong, Z., et al. 1994. Science 264: 95-98
• Shipping Conditions: Room temperature
• Storage Conditions: 4°C; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years