Anti-SOX9 / SRY-box 9 (SOX9/2398)

• Description:

  Sox genes comprise a family of genes that are related to the mammalian sex-determining gene SRY. These genes similarly contain sequences that encode for the HMG-box domain, which is responsible for the sequence-specific DNA-binding activity. Sox genes encode putative transcriptional regulators implicated in the decision of cell fates during development and the control of diverse developmental processes. SOX9 plays an important role in the normal skeletal development. It may regulate the expression of other genes involved in chondrogenesis by acting as a transcription factor for these genes.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms:

  Campomelic Dysplasia Autosomal Sex Reversal (CMD1); SRA1; SRXX2; SRY (sex determining region Y) box 9; SRY related HMG box gene 9; Transcription factor SOX9

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  SOX9

• Gene ID:

  6662

• NCBI Gene ID:

  647409

• UniProt:

  P48436

• Cellular Locus:

  Nucleus

• Host:

  Mouse

• Species Reactivity:

  Human

• Immunogen:

  Recombinant human SOX9 protein fragment (around aa 393-508) (exact sequence is proprietary)

• Target Antigen:

  SOX9 | SRY-box 9

• Clonality:

  Monoclonal

• Isotype:

  IgG1 κ

• Clone:

  SOX9/2398

• Conjugation:

  Purified, with BSA

• Source:

  Animal

• Applications:

  IHC, FFPE (verified)

• Validated Applications:

  IHC, FFPE

• Field of Research:

  Cancer, Developmental biology, Transcription factors

• Positive Control:

  HepG2 Cells. Pancreas

• Concentration:

  0.2 mg/mL

• Buffer:

  PBS, 0.05% BSA, 0.05% azide

• Molecular Weight:

  65 kDa

• Additionnal Information:

  Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunohistology (formalin): 1-2 ug/mL for 30 minutes at RT|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes|Optimal dilution for a specific application should be determined by user

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years

• CAS Number:

  9007-83-4