Anti-Parathyroid Hormone (PTH) (N-Terminal) (PTH/2295R)

Anti-Parathyroid Hormone (PTH) (N-Terminal) (PTH/2295R)

• Description: Epitope of this MAb maps in between aa 1-34. PTH is a hormone produced by the parathyroid gland that regulates the concentration of calcium and phosphorus in extracellular fluid. This hormone elevates blood Ca2 levels by dissolving the salts in bone and preventing their renal excretion. It is produced in the parathyroid gland as an 84 amino acid single chain polypeptide. It can also be secreted as N-terminal truncated fragments or C-terminal fragments after intracellular degradation, as in case of hypercalcemia. Defects in this gene are a cause of familial isolated hypoparathyroidism (FIH); also called autosomal dominant hypoparathyroidism or autosomal dominant hypocalcemia. FIH is characterized by hypocalcemia and hyperphosphatemia due to inadequate secretion of parathyroid hormone. Symptoms are seizures, tetany and cramps. FIH exist both as autosomal dominant and recessive forms of hypoparathyroidism.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
• Synonyms: hPTH; Parathormone; Parathyrin; Parathyroid hormone 1 (PTH1); Parathyroid hormone (PTH)
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: PTH
• Gene ID: 5741
• NCBI Gene ID: 37045
• UniProt: P01270
• Cellular Locus: Secreted (extracellular)
• Host: Rabbit
• Species Reactivity: Cow, Deer, Dog, Human, Mouse, Orangutan, Pig, Rabbit, Rat
• Immunogen: Synthetic peptide corresponding to amino acids 1 to 34 of mature PTH conjugated to a carrier
• Target Antigen: Parathyroid Hormone
• Clonality: Recombinant Monoclonal
• Isotype: IgG
• Clone: PTH/2295R
• Conjugation: Purified, with BSA
• Source: Animal
• Applications: IHC, FFPE (verified)
• Validated Applications: IHC, FFPE
• Field of Research: Endocrinology
• Positive Control: Human parathyroid gland carcinoma
• Concentration: 0.2 mg/mL
• Buffer: PBS, 0.05% BSA, 0.05% azide
• Molecular Weight: 9 kDa
• Additionnal Information: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunohistology (formalin): 0.25-0.5 ug/mL for 30 minutes at RT|Reactivity with non-human species predicted based on sequence|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes|Optimal dilution for a specific application should be determined by user
• Shipping Conditions: Room temperature
• Storage Conditions: 4°C; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years