Anti-S100A4 (Marker of Tumor Metastasis) (S100A4/1481)

Anti-S100A4 (Marker of Tumor Metastasis) (S100A4/1481)

• Description: S100A4 belongs to the S100 super-family of proteins containing 2 EF-hand calcium-binding domains. S100 genes include at least 25 members, including S100A1-S100A18, trichohyalin, filaggrin, repetin, S100P, and S100Z. S100A4 exerts its function via direct interaction with a number of proteins including P53, P63, non-muscle myosin IIA, alpha-6/beta-4 integrin, and liprin b1. S100A4 is overexpressed in highly metastatic cancers, which makes it useful as a marker of tumor progression._x000D_ _x000D_ Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors._x000D_ _x000D_
• Synonyms: 18A2|42A|CAPL|FSP1|Malignant transformation suppression 1 (MTS1)|Metastasin|P9KA|PEL98|Placental calcium-binding protein|S100 calcium-binding protein A4|S100A4
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: S100A4
• Gene ID: 6275
• NCBI Gene ID: 654444
• UniProt: P26447
• Cellular Locus: Nucleus & cytoplasm
• Host: Mouse
• Species Reactivity: Human, Mouse
• Immunogen: Recombinant human S100A4 protein
• Target Antigen: S100A4
• Clonality: Monoclonal
• Isotype: IgG1 κ
• Clone: S100A4/1481
• Conjugation: Purified, BSA-free
• Source: Animal
• Applications: Flow, intracellular (verified) | IF (verified) | IHC, FFPE (verified) | WB (verified)
• Validated Applications: FC, IF, IHC, FFPE, WB
• Field of Research: Cancer
• Positive Control: HeLa, A549 or A375 Cells. Placenta
• Concentration: 1 mg/mL
• Buffer: PBS, no BSA, no azide
• Molecular Weight: 10-12 kDa
• Additionnal Information: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 1-2 ug/mL|Immunohistology (formalin): 0.25-0.5 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Western blotting 0.5-1 ug/mL|Optimal dilution for a specific application should be determined by user
• Shipping Conditions: Room temperature
• Storage Conditions: -35°C to -5°C ; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years