Anti-N-Cadherin / Cadherin-2 / CD325 (NCAD) (13A9)

Anti-N-Cadherin / Cadherin-2 / CD325 (NCAD) (13A9)

• Description: This antibody recognizes a protein of ~140 kDa, identified as N-Cadherin (NCAD), also known as CD325. NCAD is a member of the Cadherin superfamily, and consists of five extracellular repeats, a transmembrane domain and a cytoplasmic domain. CD325 deficient mice die at day 10 of gestation and embryos display major heart defects and malformed neural tubes and somites. Consistent with this, CD325 has been implicated in several aspects of cardiac development including the precardiac mesoderm, establishment of left-right symmetry and cardiac looping morphogenesis. Furthermore, CD325 is normally involved in inducing cell cycle arrest and its expression is frequently deregulated in cancer cells. Studies have linked N-cadherin to cancer metastasis by showing the aggressive tumor cells had preferentially turned on N-cadherin as opposed to E- or P-cadherin.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
• Synonyms: Cadherin-2 N cadherin neuronal; Cadherin-2 type 1; Cadherin-2; Calcium dependent adhesion protein neuronal; CD325; CDH2; CDHN; CDw325; N-Cadherin; NCAD
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: CDH2
• Gene ID: 1000
• NCBI Gene ID: 464829
• UniProt: P19022
• Cellular Locus: Plasma membrane
• Host: Mouse
• Species Reactivity: Human, Mouse
• Immunogen: Recombinant human N-cadherin cytoplasmic domain (exact sequence is proprietary)
• Target Antigen: Cadherin-2 | CD325 | N-Cadherin
• Clonality: Monoclonal
• Isotype: IgG1 κ
• Clone: 13A9
• Conjugation: Purified, BSA-free
• Disease: Tumor
• Source: Animal
• Applications: IHC, FFPE (published) | IP (published) | WB (published)
• Validated Applications: IHC, FFPE, IP, WB
• Field of Research: Cancer, Cell adhesion, Developmental biology
• Positive Control: HeLa or HUVEC cells. Heart, Pancreas or Cerebral Cortex
• Concentration: 1 mg/mL
• Buffer: PBS, no BSA, no azide
• Molecular Weight: 130-140 kDa
• Additionnal Information: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 1-2 ug/mL|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Western blotting 0.5-1 ug/mL|Optimal dilution for a specific application should be determined by user
• References & Citations: Note: References for this clone sold by other suppliers may be listed for expected applications. J Biol Chem (1995) 270(34): 20201-20206. (IP; WB) J Cell Biol Volume (1996) 135(6 Pt 1): 1643-1654. (IP; WB; IF) J Histol Histopathol (2014) 1:4. http://dx.doi.org/10.7243/2055-091X-1-4 (IHC, FFPE)
• Shipping Conditions: Room temperature
• Storage Conditions: -35°C to -5°C ; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years