Anti-Histone H1 (Nuclear Marker) (r1415-1)

Anti-Histone H1 (Nuclear Marker) (r1415-1)

• Description: Eukaryotic histones are basic and water-soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed supealpha-helicalturn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
• Synonyms: H1(0)|H1.1|H1.2|H1.3|H1.4|H1.5|H1A|H1F0|H1F1|H1F2|H1F3|H1F4|H1F5|H1FNT|H1FOO|H1FT|H1FV|H1FX|H1t|H1T2|H1X|HANP1|His1|HisC|HIST1|HIST1H1A|HIST1H1B|HIST1H1C|HIST1H1D|HIST1H1E|HIST1H1T|Oocyte-specific histone H1|Testicular H1 histone
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: H1
• Gene ID: 3005
• NCBI Gene ID: 226117 & 97358
• UniProt: Multiple
• Cellular Locus: Nucleus
• Host: Mouse
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Nuclei of human leukemia biopsy cells
• Target Antigen: Histone H1
• Clonality: Recombinant Monoclonal
• Isotype: IgG2a κ
• Clone: r1415-1
• Conjugation: Purified, with BSA
• Source: Animal
• Applications: Flow, intracellular (verified) | IF (verified) | IHC, FFPE (verified) | WB (verified)
• Validated Applications: FC, IF, IHC, FFPE, WB
• Field of Research: Organelle markers
• Positive Control: HeLa, A-431, LNCap or Jurkat cells. Breast carcinoma.
• Concentration: 0.2 mg/mL
• Buffer: PBS, 0.05% BSA, 0.05% azide
• Molecular Weight: ~30 kDa
• Additionnal Information: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Immunohistology (formalin): 0.5-1 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user
• Shipping Conditions: Room temperature
• Storage Conditions: 4°C; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years