Anti-Erythropoietin (EPO) (EPO/1368)

Anti-Erythropoietin (EPO) (EPO/1368)

• Description: This antibody recognizes a protein of about 37 kDa, which is identified as Erythropoietin (EPO). Erythropoietin is a secreted, glycosylated cytokine hormone composed of four alpha helical bundles. It is the primary factor responsible for regulating erythropoiesis during steady-state conditions and in response to blood loss and hemorrhage in the adult organism. Erythropoietin is synthesized by the kidney and stimulates the proliferation and maturation of bone marrow erythroid precursor cells. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
• Synonyms: EP; EPO alpha; EPO; Epoetin; Erythropoietin; MVCD2
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: EPO
• Gene ID: 2056
• NCBI Gene ID: 2303
• UniProt: P01588
• Cellular Locus: Secreted (extracellular)
• Host: Mouse
• Species Reactivity: Human
• Immunogen: Recombinant fragment of human EPO protein (aa28-162) (exact sequence is proprietary)
• Target Antigen: Erythropoietin
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPO/1368
• Conjugation: Purified, with BSA
• Source: Animal
• Applications: IHC, FFPE (verified) | WB (verified)
• Validated Applications: IHC, FFPE, WB
• Field of Research: Cytokines, Hematology
• Positive Control: HepG2 Cells. Heart or Kidney.
• Concentration: 0.2 mg/mL
• Buffer: PBS, 0.05% BSA, 0.05% azide
• Molecular Weight: 37 kDa
• Additionnal Information: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 1-2 ug/mL|Immunohistology (formalin) 4-8 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Western blotting 0.5-1 ug/mL|Optimal dilution for a specific application should be determined by user
• Shipping Conditions: Room temperature
• Storage Conditions: 4°C; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years